Dhawale S S, Kessler K
Department of Biology, Indiana University-Purdue University, Fort Wayne 46805.
Appl Environ Microbiol. 1993 May;59(5):1675-7. doi: 10.1128/aem.59.5.1675-1677.1993.
Homokaryotic isolates of Phanerochaete chrysosporium are generally obtained by stimulating the production of basidiospores. The most commonly used method requires a special incubator that is maintained at 28 degrees C with continuous illumination. Here we report an alternate method which permits the production of basidiopores with common laboratory incubators and requires no special illumination conditions. This alternate method gives reproducible results and yields basidiospores that are not contaminated with conidia. We also report a detailed optimized method for staining basidiospores for visualizing nuclei.
黄孢原毛平革菌的同核体分离物通常通过刺激担孢子的产生来获得。最常用的方法需要一个保持在28摄氏度并持续光照的特殊培养箱。在此，我们报告一种替代方法，该方法使用普通实验室培养箱即可产生担孢子，且不需要特殊的光照条件。这种替代方法能给出可重复的结果，并产生不被分生孢子污染的担孢子。我们还报告了一种详细的优化方法，用于对担孢子进行染色以观察细胞核。
