Paoletti Andrew C, Zybailov Boris, Washburn Michael P
Stowers Institute for Medical Research, 1000 E. 50th St., Kansas City, MO 64110, USA.
Expert Rev Proteomics. 2004 Oct;1(3):275-82. doi: 10.1586/14789450.1.3.275.
Multidimensional chromatography coupled to tandem mass spectrometry is an emerging technique for the analysis of proteomes and is rapidly being implemented by many researchers for proteomic analysis. In this technology profile, a particular proteomic approach known as multidimensional protein identification technology (MudPIT) is discussed. In MudPIT, a biphasic microcapillary column is packed with high-performance liquid chromatography grade reversed phase and strong cation exchange packing materials, loaded with a complex peptide mixture and placed in line with quaternary high-performance liquid chromatography and a tandem mass spectrometer. MudPIT has the capability to analyze highly complex proteomic mixtures such as whole proteomes, organelles and protein complexes.
多维色谱联用串联质谱是一种新兴的蛋白质组分析技术，许多研究人员正迅速将其应用于蛋白质组学分析。在本技术简介中，将讨论一种特定的蛋白质组学方法，即多维蛋白质鉴定技术（MudPIT）。在MudPIT中，双相微毛细管柱填充有高效液相色谱级反相和强阳离子交换填充材料，加载复杂的肽混合物，并与四元高效液相色谱和串联质谱仪串联。MudPIT能够分析高度复杂的蛋白质组混合物，如完整蛋白质组、细胞器和蛋白质复合物。
