Qadri T, Miranda L, Tunér J, Gustafsson A
Department of Periodontology, Institute of Odontology, Karolinska Institutet, Huddinge, Sweden.
J Clin Periodontol. 2005 Jul;32(7):714-9. doi: 10.1111/j.1600-051X.2005.00749.x.
The aim of this split-mouth, double-blind controlled clinical trial was to study the effects of irradiation with low-level lasers as an adjunctive treatment of inflamed gingival tissue.
Seventeen patients with moderate periodontitis were included. After clinical examination, all teeth were scaled and root planed (SRP). One week after SRP, we took samples of gingival crevicular fluid (GCF) and subgingival plaque. The laser therapy was started 1 week later and continued once a week for 6 weeks. One side of the upper jaw was treated with active laser and the other with a placebo. The test side was treated with two low-level lasers having wavelengths of 635 and 830 nm. The patients then underwent another clinical examination with sampling of GCF and plaque. The GCF samples were analysed for elastase activity, interleukin-1beta (IL-1beta) and metalloproteinase-8 (MMP-8). We examined the subgingival plaque for 12 bacteria using DNA probes.
The clinical variables i.e. probing pocket depth, plaque and gingival indices were reduced more on the laser side than on the placebo one (p<0.01). The decrease in GCF volume was also greater on the laser side, 0, 12 microl, than on the placebo side, 0.05 microl (p=0.01). The total amount of MMP-8 increased on the placebo side but was slightly lower on the laser side (p=0.052). Elastase activity, IL-1beta concentration and the microbiological analyses showed no significant differences between the laser and placebo sides.
Additional treatment with low-level lasers reduced periodontal gingival inflammation.
本双盲对照临床试验旨在研究低强度激光照射作为炎症牙龈组织辅助治疗的效果。
纳入17例中度牙周炎患者。临床检查后，对所有牙齿进行龈下刮治和根面平整（SRP）。SRP术后1周，采集龈沟液（GCF）和龈下菌斑样本。1周后开始激光治疗，每周1次，持续6周。上颌一侧用活性激光治疗，另一侧用安慰剂治疗。试验侧用两台波长分别为635和830 nm的低强度激光治疗。患者随后接受另一次临床检查，采集GCF和菌斑样本。对GCF样本进行弹性蛋白酶活性、白细胞介素-1β（IL-1β）和基质金属蛋白酶-8（MMP-8）分析。我们使用DNA探针检测龈下菌斑中的12种细菌。
临床变量，即探诊深度、菌斑和牙龈指数，激光治疗侧比安慰剂侧降低得更多（p<0.01）。激光治疗侧GCF体积的减少量也大于安慰剂侧，分别为0.12微升和0.05微升（p=0.01）。安慰剂侧MMP-8总量增加，但激光治疗侧略低（p=0.052）。弹性蛋白酶活性、IL-1β浓度和微生物学分析显示，激光治疗侧与安慰剂侧之间无显著差异。
低强度激光辅助治疗可减轻牙周牙龈炎症。
