Fegou E, Jelastopulu E, Sevdali M, Anastassiou E D, Dimitracopoulos G, Spiliopoulou I
Department of Microbiology, School of Medicine, University of Patras, Greece.
Clin Microbiol Infect. 2005 Jul;11(7):593-6. doi: 10.1111/j.1469-0691.2005.01185.x.
The Cobas Amplicor PCR system (CA-PCR) was compared with culture and staining for acid-fast bacilli (AFB) for the early detection of Mycobacterium tuberculosis in respiratory clinical specimens and otherwise normal sterile body fluids. The sensitivity, specificity and positive and negative predictive values of CA-PCR were determined with AFB-positive and AFB-negative specimens. The sensitivity of CA-PCR ranged from 73.6% to 100% for AFB-positive samples, while sputa collected after bronchoscopy were the most useful specimens, with 70% sensitivity and 98.6% specificity among the AFB-negative samples.
将Cobas Amplicor聚合酶链反应（PCR）系统（CA-PCR）与抗酸杆菌（AFB）培养及染色法进行比较，以用于呼吸道临床标本及其他正常无菌体液中结核分枝杆菌的早期检测。通过AFB阳性和AFB阴性标本确定CA-PCR的敏感性、特异性以及阳性和阴性预测值。对于AFB阳性样本，CA-PCR的敏感性在73.6%至100%之间，而支气管镜检查后采集的痰液是最有用的标本，在AFB阴性样本中敏感性为70%，特异性为98.6%。
