Alfaresi Mubarak S, Abdulsalam Adeel I, Elkoush Abida A
Department of Microbiology, Zayed Military Hospital, Abu Dhabi, United Arab Emirates.
Saudi Med J. 2006 Sep;27(9):1346-51.
To evaluate the recently available Cobas Amplicor polymerase chain reaction (PCR) system for the detection of Mycobacterium tuberculosis complex (MTBC) in well-characterized clinical specimens and to compare the results with clinical classification, conventional culture and staining techniques.
Three hundred and forty-four clinical specimens consecutively received for culture of acid-fast bacilli by the laboratory of Zayed Military Hospital, Abu Dhabi, United Arab Emirates, from 2004 to 2005, were used in this study. Acid-fast bacilli and culture for tuberculosis were compared with Cobas Amplicor PCR.
The final diagnostic evaluation of the Cobas Amplicor MTB showed the followings: sensitivity 73.3%, specificity 99.4%, positive predictive value 84.6%, negative predictive value 98.8% and with accuracy of 98.2% for the Cobas Amplicor MTB assay.
The automated Cobas Amplicor MTB assay is suitable for routine use in clinical laboratories.
评估最近可用的 Cobas Amplicor 聚合酶链反应（PCR）系统，用于检测特征明确的临床标本中的结核分枝杆菌复合群（MTBC），并将结果与临床分类、传统培养和染色技术进行比较。
本研究使用了 2004 年至 2005 年期间阿联酋阿布扎比扎耶德军事医院实验室连续接收的 344 份用于抗酸杆菌培养的临床标本。将抗酸杆菌和结核培养结果与 Cobas Amplicor PCR 进行比较。
Cobas Amplicor MTB 的最终诊断评估结果如下：Cobas Amplicor MTB 检测的灵敏度为 73.3%，特异性为 99.4%，阳性预测值为 84.6%，阴性预测值为 98.8%，准确率为 98.2%。
自动化的 Cobas Amplicor MTB 检测适用于临床实验室的常规使用。
