Kucera R, Sochor J, Klimes J, Dohnal J
Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control, Heyrovského 1203, CZ-50005 Hradec Králové, Czech Republic.
J Pharm Biomed Anal. 2005 Jul 15;38(4):609-18. doi: 10.1016/j.jpba.2005.02.002. Epub 2005 Mar 17.
A new reversed-phase liquid chromatographic method using zirconia-based stationary phase was developed for determination of ibuprofen, its related compounds and its main degradation products. The chromatographic separation was successfully achieved on the Discovery Zr-PS column (150 mm x 4.6 mm i.d., 5 microm), using a mobile phase methanol-phosphate buffer (pH 4.5; 0.05 M)-tetrahydrofurane (21:74:5, v/v/v) and the flow rate 0.5 ml min(-1). The UV detection was performed in dual wavelength mode (219 and 258 nm) to detect all compounds of interest. The column temperature was set on 60 degrees C to shorten the analysis time and improve the peak symmetry. The method is simple, rapid and cuts down the amount of hazardous waste produced in the analysis. The assay is completed within 22 minutes.
开发了一种使用氧化锆基固定相的新型反相液相色谱法，用于测定布洛芬、其相关化合物及其主要降解产物。在Discovery Zr-PS柱（150 mm×4.6 mm内径，5微米）上成功实现了色谱分离，流动相为甲醇 - 磷酸盐缓冲液（pH 4.5；0.05 M） - 四氢呋喃（21:74:5，v/v/v），流速为0.5 ml min⁻¹。采用双波长模式（219和258 nm）进行紫外检测，以检测所有目标化合物。柱温设定为60℃，以缩短分析时间并改善峰形对称性。该方法简单、快速，减少了分析过程中产生的有害废物量。测定在22分钟内完成。
