Fu Wenxian, Zhang Yu, Zhang Jun, Chen Wei-Feng
Department of Immunology, Peking University Health Science Center, Beijing 100083, China.
Cytokine. 2005 Jul 7;31(1):9-17. doi: 10.1016/j.cyto.2005.02.005. Epub 2005 Apr 20.
CXCR2/IL-8RB was the only receptor previously reported in mice for ELR+ CXC chemokines, whereas the receptors for these chemokines in human include both CXCR1 and CXCR2. In this study, we cloned the full length cDNA of the mouse CXCR1 (mCXCR1) gene. The deduced amino acid of mCXCR1 was 77% and 58% identical to the rat and human CXCR1, respectively. RT-PCR and Northern blot analysis showed that mCXCR1 mRNA was expressed in lung, spleen, thymus, peripheral blood leukocytes, as well as in the isolated neutrophils. In a mouse respiratory inflammation model induced by lipopolysaccharide, a large number of neutrophils infiltrated into the lung and, meanwhile, the mCXCR1 expression was significantly increased in the recruited neutrophils, suggesting that mCXCR1 may mediate the recruitment of neutrophils to the inflammation site under certain infections.
CXCR2/IL-8RB是先前报道的小鼠中唯一针对ELR+CXC趋化因子的受体，而人类中这些趋化因子的受体包括CXCR1和CXCR2。在本研究中，我们克隆了小鼠CXCR1（mCXCR1）基因的全长cDNA。推导的mCXCR1氨基酸序列与大鼠和人类CXCR1的同源性分别为77%和58%。RT-PCR和Northern印迹分析表明，mCXCR1 mRNA在肺、脾、胸腺、外周血白细胞以及分离的中性粒细胞中表达。在脂多糖诱导的小鼠呼吸道炎症模型中，大量中性粒细胞浸润到肺中，同时，募集到的中性粒细胞中mCXCR1表达显著增加，这表明mCXCR1可能在某些感染情况下介导中性粒细胞向炎症部位的募集。
