Gardocki Mary E, Jani Niketa, Lopes John M
Department of Biological Sciences, Wayne State University, 5047 Gullen Mall, Detroit MI 48202, USA.
Biochim Biophys Acta. 2005 Jul 15;1735(2):89-100. doi: 10.1016/j.bbalip.2005.05.006.
Phosphatidylinositol (PI) is a ubiquitous membrane lipid in eukaryotes. It is becoming increasingly obvious that PI and its metabolites play a myriad of very diverse roles in eukaryotic cells. The Saccharomyces cerevisiae PIS1 gene is essential and encodes PI synthase, which is required for the synthesis of PI. Recently, PIS1 expression was found to be regulated in response to carbon source and oxygen availability. It is particularly significant that the promoter elements required for these responses are conserved evolutionarily throughout the Saccharomyces genus. In addition, several genome-wide strategies coupled with more traditional screens suggest that several other factors regulate PIS1 expression. The impact of regulating PIS1 expression on PI synthesis will be discussed along with the possible role(s) that this may have on diseases such as cancer.
磷脂酰肌醇（PI）是真核生物中普遍存在的膜脂。越来越明显的是，PI及其代谢产物在真核细胞中发挥着无数非常多样的作用。酿酒酵母PIS1基因是必需的，编码PI合酶，这是PI合成所必需的。最近，发现PIS1表达受碳源和氧气供应的调控。特别重要的是，这些反应所需的启动子元件在整个酵母属中进化上是保守的。此外，几种全基因组策略与更传统的筛选方法表明，其他几个因素也调节PIS1表达。将讨论调节PIS1表达对PI合成的影响以及这可能对癌症等疾病产生的潜在作用。
