Badia E, Pinart E, Briz M, Pastor L M, Sancho S, Garcia-Gil N, Bassols J, Kádár E, Pruneda A, Bussalleu E, Yeste M, Bonet S
Biotechnology of Porcine Reproduction, Department of Biology, Faculty of Sciences, University of Girona, Campus de Montilivi, Girona, Spain.
Eur J Histochem. 2005 Apr-Jun;49(2):131-8.
The present study describes, for the first time, the glycosidic content of boar bulbourethral glands using lectin histochemistry. Fourteen horseradish peroxidase- or digoxigenin-labelled lectins with different carbohydrate specificities were used in samples obtained from 3 healthy Landrace boars. The results obtained indicate that endpiece and duct cells synthesize and secrete mainly O-glycoproteins with alpha- and beta-D-N-acetylgalactosamine, beta-D-galactose-beta(1-->3)-D-N-acetylgalactosamine, D-N-acetylglucosamine and neuraminic acid residues. Glycoproteins secreted by bulbourethral glands have a role in the protection and lubrication of the urethra. In addition, they may be also involved in the regulation of the sperm metabolic activity and in the maintenance of the structural integrity of acrosomal and plasma membranes.
本研究首次利用凝集素组织化学描述了公猪尿道球腺的糖苷含量。从3头健康的长白公猪获取样本，使用了14种具有不同碳水化合物特异性的辣根过氧化物酶或地高辛标记凝集素。所得结果表明，终末段和导管细胞主要合成和分泌含有α-和β-D-N-乙酰半乳糖胺、β-D-半乳糖-β(1→3)-D-N-乙酰半乳糖胺、D-N-乙酰葡糖胺和神经氨酸残基的O-糖蛋白。尿道球腺分泌的糖蛋白对尿道具有保护和润滑作用。此外，它们可能还参与精子代谢活性的调节以及顶体膜和质膜结构完整性的维持。
