Ohta Yoshiki, Suzuki Takao, Shiokawa Akira, Mitsuya Toshiyuki, Ota Hidekazu
Department of Pathology, Showa University Northern Yokohama Hospital, Kanagawa 227-8503, Japan.
Int J Gynecol Pathol. 2005 Jul;24(3):239-45. doi: 10.1097/01.pgp.0000158533.28866.45.
A solid pattern of tumor cells with a clear cytoplasm is common to both ovarian clear cell carcinoma (OvCCC) and renal clear cell carcinoma (RCCC). This study examined the possible differential expression of CD10 and cytokeratins (CK7, CK20, 34betaE12, and CAM5.2) between these two types. An immunohistochemical technique using peroxidase-labeled amino acid polymers was used to test formalin-fixed and paraffin-embedded tissues. In OvCCC, 6 of 29 cases were positive for CD10, and all cases had expression of CK7, 34betaE12, and CAM5.2. In contrast, all 24 RCCC cases had CD10 and CAM5.2 immunoreactivity, but none had any staining for 34betaE12. CK7 was only expressed in nine cases. No CK20 positivity was observed in any sample from either tumor type. Localization of CD10 expression was different in OvCCC versus RCCC. Although positive staining for 34betaE12 strongly suggests OvCCC, sometimes only a few cells may be stained. Therefore, 34betaE12-negative biopsies also should be evaluated for CD10 and CK7 immunoreactivity to enable histologic and cytologic differential diagnosis.
肿瘤细胞呈实性排列且胞质透明，这在卵巢透明细胞癌（OvCCC）和肾透明细胞癌（RCCC）中都很常见。本研究检测了这两种类型肿瘤之间CD10和细胞角蛋白（CK7、CK20、34βE12和CAM5.2）的可能差异表达。采用过氧化物酶标记氨基酸聚合物的免疫组织化学技术检测福尔马林固定、石蜡包埋的组织。在OvCCC中，29例中有6例CD10呈阳性，所有病例均表达CK7、34βE12和CAM5.2。相比之下，24例RCCC病例CD10和CAM5.2均呈免疫反应性，但均无34βE12染色。CK7仅在9例中表达。两种肿瘤类型的任何样本中均未观察到CK20阳性。OvCCC和RCCC中CD10表达的定位不同。虽然34βE12阳性染色强烈提示为OvCCC，但有时可能只有少数细胞被染色。因此，对于34βE12阴性的活检组织，也应评估CD10和CK7的免疫反应性，以进行组织学和细胞学鉴别诊断。
