Wu Xiao-jiang, Wang Yu, Ji Jia-fu
Department of Surgery, Peking University School of Oncology, Beijing Cancer Hospital, Beijing 100036, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2005 Jun 18;37(3):252-6.
To study the expression of 11 cancer-testis (CT) genes and autologous humoral immune response to NY-ESO-1 in gastric carcinoma in China to provide some data for developing a vaccine against this cancer.
Pathology tests were conducted in 101 gastric carcinoma samples, including tumor tissue and paired adjacent normal non-tumor tissue. Reverse transcription-polymerase chain reaction (RT-PCR) was used to test for 11 CT antigen gene expression and ELISA for autologous humoral immune response to NY-ESO-1. The antigen of NY-ESO-1 was also detected in tumor tissues by mAb E978 in IHC.
About 74.3% of the samples expressed at least one CT antigen, and the most frequently expressed one was MAGE-3 (41.6%). Twelve out of the 101 samples (11.9%) were found to be NY-ESO-1 mRNA positive. Simultaneously, autologous humoral immune response to NY-ESO-1 was detected in 6 of 12 NY-ESO-1 mRNA positive patients.
Multiple CT genes were expressed in gastric cancer samples. MAGE-3, SSX-1, SSX-4, NY-ESO-1/LAGE-1, and MAGE-1 can be chosen as candidate antigens for cancer vaccines due to their higher expression rate. The potential of a multivalent CT antigen vaccine, including NY-ESO-1 and MAGE-3 along with other CT antigens, should be evaluated in patients with gastric cancer.
研究11种癌-睾丸（CT）基因在中国胃癌中的表达以及对NY-ESO-1的自体体液免疫反应，为开发针对该癌症的疫苗提供一些数据。
对101例胃癌样本进行病理检测，包括肿瘤组织和配对的相邻正常非肿瘤组织。采用逆转录-聚合酶链反应（RT-PCR）检测11种CT抗原基因的表达，酶联免疫吸附测定（ELISA）检测对NY-ESO-1的自体体液免疫反应。还通过免疫组化法用单克隆抗体E978检测肿瘤组织中的NY-ESO-1抗原。
约74.3%的样本至少表达一种CT抗原，最常表达的是MAGE-3（41.6%）。101个样本中有12个（11.9%）被发现NY-ESO-1 mRNA呈阳性。同时，在12例NY-ESO-1 mRNA阳性患者中的6例检测到对NY-ESO-1的自体体液免疫反应。
多种CT基因在胃癌样本中表达。由于其较高的表达率，MAGE-3、SSX-1、SSX-4、NY-ESO-1/LAGE-1和MAGE-1可作为癌症疫苗的候选抗原。应在胃癌患者中评估包含NY-ESO-1和MAGE-3以及其他CT抗原的多价CT抗原疫苗的潜力。
