Gao Min, Wei Li-hui, Sun Peng-ming, Wang Jian-liu, Zhao Dan, Zhao Chao, Wang Zhi-qi
Department of Gynecology, Peking University People's Hospital, Beijing 100044, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2005 Jun 18;37(3):281-3.
To explore the relationship between expression of ERR alpha mRNA and estrogen and progesterone and to elucidate the function of ERR alpha in endometrial carcinoma.
The expression of ERR alpha mRNA was examined by reverse transcription polymerase chain reaction. Endometrial carcinoma cell line Ishikawa was dealt with different concentrations of 17beta-estradiol (10(-10) mol/L, 10(-8) mol/L and 10(-6) mol/L) for 15 min, 30 min and 24 h and 17beta-estradiol and ER inhibitor-ICI182780 were given concomitantly to observe the change of ERR alpha mRNA. Different concentrations of progesterone (10(-8) mol/L, 10(-7) mol/L, 10(-6) mol/L and 10(-5) mol/L) were also given to Ishikawa cell line for 24 h to observe the change of ERR alpha mRNA.
The expression level of ERR alpha mRNA was slightly higher than that of the control group after being stimulated for 15 min, 30 min and 24 h by 10(-10) mol/L 17beta-estradiol. However the expression level of ERR alpha mRNA was significantly lower than that of the control group after being stimulated for 15 min, 30 min and 24 h by 10(-8) mol/L and 10(-6) mol/L of 17beta-estradiol. When 10(-6) mol/L of ICI182780 and 10(-8) mol/L of 17beta-estradiol were given simultaneously to Ishikawa cell line, this down-regulation was blocked. After being stimulated for 24 h by 10(-7) mol/L, 10(-6) mol/L and 10(-5) mol/L of progesterone, the expression levels of ERR alpha mRNA were significantly higher than that of the control group, but no change was found in 10(-8) mol/L of progesterone.
17Beta-estradiol can down-regulate the expression of ERR alpha mRNA and this regulation is mediated by estrogen receptor. Progesterone can up-regulate the expression of ERR alpha mRNA.
探讨雌激素相关受体α（ERRα）mRNA表达与雌激素、孕激素的关系，阐明ERRα在子宫内膜癌中的作用。
采用逆转录聚合酶链反应检测ERRα mRNA的表达。用不同浓度的17β-雌二醇（10⁻¹⁰mol/L、10⁻⁸mol/L和10⁻⁶mol/L）处理子宫内膜癌细胞系Ishikawa 15分钟、30分钟和24小时，并同时给予17β-雌二醇和雌激素受体抑制剂ICI182780，观察ERRα mRNA的变化。还用不同浓度的孕激素（10⁻⁸mol/L、10⁻⁷mol/L、10⁻⁶mol/L和10⁻⁵mol/L）处理Ishikawa细胞系24小时，观察ERRα mRNA的变化。
10⁻¹⁰mol/L 17β-雌二醇刺激15分钟、30分钟和24小时后，ERRα mRNA表达水平略高于对照组。然而，10⁻⁸mol/L和10⁻⁶mol/L 17β-雌二醇刺激15分钟、30分钟和24小时后，ERRα mRNA表达水平显著低于对照组。当向Ishikawa细胞系同时给予10⁻⁶mol/L ICI182780和10⁻⁸mol/L 17β-雌二醇时，这种下调被阻断。10⁻⁷mol/L、10⁻⁶mol/L和10⁻⁵mol/L孕激素刺激24小时后，ERRα mRNA表达水平显著高于对照组，但10⁻⁸mol/L孕激素处理后未发现变化。
17β-雌二醇可下调ERRα mRNA的表达，且该调节由雌激素受体介导。孕激素可上调ERRα mRNA的表达。
