Lu Ye, Yu Li, Chen Chun-ling, Liao Qin-ping
Department of Obstetrics & Gynecology, Peking University First Hospital, Beijing 100034, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2005 Jun 18;37(3):284-6.
To study the effects of estrogen, progestogen and mifepristone on endometrial carcinoma cell lines in vitro.
The well-differentiated endometrial cancer cell line Ishikawa and moderate-differentiated endometrial cancer cell line Hec-1B were cultured in vitro. The cells were divided into four groups: control, estrogen, estrogen and progestogen, estrogen and progestogen and mifepristone, then we implanted cells in 96-well plates to search the response in distinct hormones.
When stimulated by estrogen for 72 hours, the growth of Ishikawa cells was significantly higher than the control, Hec-1B cells only grew higher than the control, but it was no significance in statistics. Progestogen inhibited Ishikawa cells significantly after being stimulating for 72 hours, there was the same effect on Hec-1B cells after being stimulating for 96 hours. On the base of estrogen and progestogen, we added mifepristone, cells developed after 96 hours in Ishikawa cells and cells developed after 48 hours in Hec-1B cells.
Estrogen can cause endometrial carcinoma cell growth; progestogen inhibits the hyperplasia induced by estrogen; mifepristone antagonizes the effect of progestogen on cell growth.
研究雌激素、孕激素及米非司酮对子宫内膜癌细胞系的体外作用。
体外培养高分化子宫内膜癌细胞系Ishikawa和中分化子宫内膜癌细胞系Hec-1B。将细胞分为四组：对照组、雌激素组、雌激素加孕激素组、雌激素加孕激素加米非司酮组，然后将细胞接种于96孔板中，观察不同激素作用下的反应。
雌激素作用72小时后，Ishikawa细胞生长明显高于对照组，Hec-1B细胞仅生长高于对照组，但差异无统计学意义。孕激素作用72小时后对Ishikawa细胞有明显抑制作用，作用96小时后对Hec-1B细胞有同样作用。在雌激素和孕激素基础上加入米非司酮，Ishikawa细胞96小时后生长，Hec-1B细胞48小时后生长。
雌激素可致子宫内膜癌细胞生长；孕激素抑制雌激素诱导的增生；米非司酮拮抗孕激素对细胞生长的作用。
