Yang Yu, Wang Hua, Wang Yu-ping, Zhang Wei, Hui Yun-zhong, Wang Zi-hui, Zheng Jie
Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100083, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2005 Jun 18;37(3):325-8.
To detect the EWS-WT1 chimeric mRNA in desmoplastic small round cell tumor (DSRCT) and its clinicopathological significance.
Formalin fixed, paraffin-embedded tumor specimens from 4 examples of this entity were studied by reverse transcriptase polymerase chain reaction to detect the EWS-WT1 fusion transcripts resulting from the chromosomal translocation t(11;22)(p13;q12). The following tumor specimens were included as controls: 2 Ewing's sarcomas/primitive neuroectodermal tumors (PNET/ES), 2 alveolar rhabdomyosarcomas, and 2 lymphomas.
EWS-WT1 fusion transcripts were detected in 3 of 4 desmoplastic small round cell tumors but not in any other tumor types studied as controls.
The analysis of the EWS-WT1 fusion transcript which was performed on formalin-fixed, paraffin-embedded tissues is a sensitive and specific method in the diagnosis and differential diagnosis of DSRCT.
检测促纤维组织增生性小圆细胞肿瘤（DSRCT）中的EWS-WT1嵌合mRNA及其临床病理意义。
采用逆转录聚合酶链反应研究4例该实体瘤经福尔马林固定、石蜡包埋的肿瘤标本，以检测由染色体易位t(11;22)(p13;q12)产生的EWS-WT1融合转录本。以下肿瘤标本作为对照：2例尤因肉瘤/原始神经外胚层肿瘤（PNET/ES）、2例肺泡横纹肌肉瘤和2例淋巴瘤。
4例促纤维组织增生性小圆细胞肿瘤中有3例检测到EWS-WT1融合转录本，而在作为对照研究的其他任何肿瘤类型中均未检测到。
对福尔马林固定、石蜡包埋组织进行EWS-WT1融合转录本分析是DSRCT诊断及鉴别诊断中的一种敏感且特异的方法。
