Inhibition of penta-acetyl geniposide on AFB1-induced genotoxicity in C3H10T1/2 cells.

A new compound, penta-acetyl geniposide ((Ac)5-GP), was obtained from modified extract of Gardenia fructus (San-Jee-Chee in Chinese). The structure of the compound was identified as 1-(beta-D-2',3',4',6'-tetraacetyl-glucopyrannosyloxyl)-1,4a, 5,7a-tetrahydro-7-(acetomethyl)-cyclopentapyran-4-carboxylic acid methyl ester, according to the spectral data. The inhibitory effects of (Ac)5-GP on aflatoxin B1 (AFB1)-induced cytotoxicity and DNA damage were studied. In the investigation of the inhibitory effect of (Ac)5-GP on AFB1-cytotoxicity, the plating efficiency of C3H10T1/2 cells in S-9 activation system was increased. In addition, (Ac)5-GP inhibited the DNA damage of AFB1-treated C3H10T1/2 cells, and it interfered with the inhibitory effect of DNA synthesis caused by AFB1. These results suggest that the reduced DNA damage and the increased DNA synthesis from cultured C3H10T1/2 cells are important mechanisms for the inhibition of AFB1-cytotoxicity by (Ac)5-GP.