Yu Wei-Dong, Li Wen-Yong, Wang Yu-Ge, Yang Li-Xin, Liu Gui-Sheng, Du Miao, Chen Qing-Xuan
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Graduated School of Chinese Academy of Sciences, Beijing 100080, China.
Sheng Wu Gong Cheng Xue Bao. 2003 Jan;19(1):30-4.
By the method of single preimplantation embryos differential display polymerase chain reaction (SPEDDRT-PCR), 25 reprogramming cDNA fragments were obtained from single 2-cell, 8-cell embryos and blastula. After cloning and sequencing, five of them were identified by reverse-Northern and characterized with stage-specific expression during reconstructed embryo development. This results will help to isolate full length reprogramming genes and study their function during embryonic development.
通过单胚胎植入前差异显示聚合酶链反应（SPEDDRT-PCR）方法，从单个2细胞、8细胞胚胎和囊胚中获得了25个重编程cDNA片段。经过克隆和测序后，其中5个通过反向Northern杂交进行鉴定，并对其在重构胚胎发育过程中的阶段特异性表达进行了表征。这些结果将有助于分离全长重编程基因并研究它们在胚胎发育过程中的功能。
