Mernaugh R L, Shearer M H, Bright R K, Lanford R E, Kennedy R C
Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX 78228.
Cancer Immunol Immunother. 1992;35(2):113-8. doi: 10.1007/BF01741858.
Baculovirus-derived recombinant simian virus 40 (SV40) large tumor antigen (SV40 T-Ag), a monoclonal antibody specific for SV40 T-Ag (Ab-1 preparation), and a monoclonal anti-idiotypic antibody (anti-Id), designated 58D, were used to analyze the humoral immune response of Balb/c mice either immunized with recombinant SV40 T-Ag or challenged with SV40-transformed cells. Inhibition assays indicated that antibodies from mice immunized with SV40 T-Ag and from those bearing SV40 tumor inhibited the SV40 T-Ag/Ab-1 reaction. These data suggested that the antibody response in immunized or tumor-challenged mice recognized similar epitope(s) on SV40 T-Ag to that detected by the monoclonal Ab-1. These anti-(SV40 T-Ag) response antibodies also inhibited the Ab-1/anti-Id reaction and recognized the anti-Id in direct binding assays. Together, these data indicate that murine anti-(SV40 T-Ag) responses shared an idiotope with a monoclonal anti-(SV40 T-Ag) Ab-1 preparation. This idiotope, which is recognized by the monoclonal anti-Id preparation, 58D, appears to be involved in the humoral immune response to SV40 T-Ag in both SV40-T-Ag-immunized and tumor-bearing mice. The monoclonal anti-Id preparation may represent a focal point for manipulating the humoral immune response to tumors induced by SV40-transformed cells.
杆状病毒衍生的重组猴病毒40（SV40）大T抗原（SV40 T-Ag）、一种对SV40 T-Ag具有特异性的单克隆抗体（Ab-1制剂）以及一种指定为58D的单克隆抗独特型抗体（抗Id），被用于分析用重组SV40 T-Ag免疫或用SV40转化细胞攻击的Balb/c小鼠的体液免疫反应。抑制试验表明，来自用SV40 T-Ag免疫的小鼠和携带SV40肿瘤的小鼠的抗体抑制了SV40 T-Ag/Ab-1反应。这些数据表明，免疫或肿瘤攻击小鼠中的抗体反应识别的SV40 T-Ag上的表位与单克隆抗体Ab-1检测到的相似。这些抗（SV40 T-Ag）反应抗体也抑制了Ab-1/抗Id反应，并在直接结合试验中识别抗Id。这些数据共同表明，小鼠抗（SV40 T-Ag）反应与单克隆抗（SV40 T-Ag）Ab-1制剂共享一个独特型。这个独特型被单克隆抗Id制剂58D识别，似乎在SV40-T-Ag免疫小鼠和荷瘤小鼠中对SV40 T-Ag的体液免疫反应中起作用。单克隆抗Id制剂可能是操纵对SV40转化细胞诱导的肿瘤的体液免疫反应的一个焦点。
