Beacham Dorothy A, Cukierman Edna
Fox Chase Cancer Center, Basic Science/Tumor Cell Biology, 333 Cottman Avenue, Philadelphia, PA 19111-2497, USA.
Semin Cancer Biol. 2005 Oct;15(5):329-41. doi: 10.1016/j.semcancer.2005.05.003.
During tumorigenesis, reciprocal changes in stromal fibroblasts and tumor cells induce changes to the neoplastic microenvironmental landscape. In stromagenesis, both the complex network of bi-directional stromal fibroblastic signaling pathways and the stromal extracellular matrix are modified. The presence of a 'primed' stroma during the early, reversible stage of tumorigenesis is optimal for stromal-directed therapeutic intervention. Three-dimensional (3D) cell culture systems have been developed that mimic the in vivo microenvironment. These systems provide unique experimental tools to identify early alterations in stromagenesis that are supportive of tumor progression with the ultimate goal of blocking neoplastic permissiveness and restoring normal phenotypes.
在肿瘤发生过程中，基质成纤维细胞和肿瘤细胞的相互变化会诱导肿瘤微环境格局的改变。在基质形成过程中，双向基质成纤维细胞信号通路的复杂网络和基质细胞外基质都会发生改变。在肿瘤发生的早期可逆阶段存在“预激”基质，这对于基质导向的治疗干预是最佳的。已经开发出模拟体内微环境的三维（3D）细胞培养系统。这些系统提供了独特的实验工具，以识别基质形成中支持肿瘤进展的早期改变，最终目标是阻断肿瘤的易感性并恢复正常表型。
