Zhai Xin-Hui, Xing Pei-Ni, Wei Xu-Cang, Zhao Wen-Li, Li Mei-Sheng
Department of Hematology, Shanxi Province People Hospital, Xi' an 710068, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2005 Jun;13(3):488-91.
To study the influence of IFN-alpha on function of CML-DC cultured in vitro and expression of chemokine and its chemokine receptor, bone marrow mononuclear cells from 13 CML patients were cultured in the fetal calf serum culture system supplemented with rhSCF, rhFlt-3L for expansion system, and adding rhGM-CSF, rhTNF-alpha, rhIL-4, with or without rhIFN-alpha to induce DCs. After incubation for two weeks, the phenotypes of CML-DC were analyzed by direct immunofluorescence and flow cytometry. The concentration of MIP-3beta expressed by CML-DC in the supernatant were analyzed by ELISA. The proliferative ability of T cells from healthy volunteers stimulated by CML-DCs were measured by MTT assay. The results showed that expression of CD86, CD83, CD40, MHC-I class molecules, CCR7, the concentration of MIP-3beta expressed by CML-DC, and the proliferative ability of T cells stimulated by CML-DCs in IFN-alpha group were all significantly higher than that in control group (P < 0.01). It is concluded that the immunophenotype of CML-DCs can be partially changed by IFN-alpha to accelerate the maturation of CML-DCs, enhance the capacity of CML-DCs, and stimulate allogeneic T lymphocyte proliferation.
为研究干扰素-α（IFN-α）对体外培养的慢性粒细胞白血病树突状细胞（CML-DC）功能、趋化因子及其趋化因子受体表达的影响，取13例慢性粒细胞白血病患者的骨髓单个核细胞，在含重组人干细胞因子（rhSCF）、重组人Flt-3配体（rhFlt-3L）的胎牛血清培养体系中进行扩增培养，加入重组人粒细胞巨噬细胞集落刺激因子（rhGM-CSF）、重组人肿瘤坏死因子-α（rhTNF-α）、重组人白细胞介素-4（rhIL-4），分别加入或不加入rhIFN-α诱导生成树突状细胞。培养两周后，采用直接免疫荧光法和流式细胞术分析CML-DC的表型。采用酶联免疫吸附测定法（ELISA）分析CML-DC在上清液中表达的巨噬细胞炎性蛋白-3β（MIP-3β）浓度。采用噻唑蓝（MTT）比色法检测健康志愿者T细胞经CML-DC刺激后的增殖能力。结果显示，IFN-α组CML-DC的CD86、CD83、CD40、MHC-Ⅰ类分子、CCR7的表达、CML-DC表达的MIP-3β浓度以及CML-DC刺激的T细胞增殖能力均显著高于对照组（P<0.01）。结论：IFN-α可部分改变CML-DC的免疫表型，加速CML-DC成熟，增强CML-DC功能，刺激异体T淋巴细胞增殖。
