米曲霉的果糖基氨基酸氧化酶由反应产物葡糖酮诱导产生。
Fructosyl-amino acid oxidases of Aspergillus oryzae are induced by the reaction product, glucosone.
作者信息
Yoshida Nobuyuki, Akazawa Shin-ichi, Kuwahara Ayako, Katsuragi Tohoru, Tani Yoshiki
机构信息
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma 630-0192, Japan.
出版信息
FEMS Microbiol Lett. 2005 Jul 15;248(2):141-5. doi: 10.1016/j.femsle.2005.05.035.
Aspergillus oryzae has two fructosyl-amino acid oxidase (FAOD) isozymes (AoFao1 and AoFao2), which are different in the substrate specificities. Northern blot analysis showed both FAO genes were induced by autoclave-browned medium containing l-lysine or l-valine. Studies with a mutant, that had a disrupted AoFAO2 gene, revealed that the expression of AoFAO1 by fructosyl l-valine depended on the expression of AoFAO2. Both genes were also induced by one of the FAOD-reaction products, glucosone. In contrast, other alpha-dicarbonyl compounds, which display a similar structure to that of glucosone were not able to induce the genes expression. These results imply that glucosone may contribute to the expression of FAO genes.
米曲霉有两种果糖基氨基酸氧化酶（FAOD）同工酶（AoFao1和AoFao2），它们的底物特异性不同。Northern印迹分析表明，这两个FAO基因均由含有L-赖氨酸或L-缬氨酸的高压灭菌褐变培养基诱导。对一个AoFAO2基因被破坏的突变体的研究表明，果糖基L-缬氨酸对AoFAO1的表达依赖于AoFAO2的表达。这两个基因也都被FAOD反应产物之一葡糖酮诱导。相比之下，其他与葡糖酮结构相似的α-二羰基化合物不能诱导基因表达。这些结果表明葡糖酮可能有助于FAO基因的表达。
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