Qiu J, Beckman M J, Qian J, Jiranek W
Department of Orthopaedic Surgery, Orthopaedic Research Laboratory, Virginia Commonwealth University Medical Center, Richmond, VA 23298-0694, USA.
J Orthop Res. 2005 Jul;23(4):942-8. doi: 10.1016/j.orthres.2005.04.008.
Human mast cells (MC) exhibit two distinct phenotypes based on the protease content of their secretory granules. MC(TC) cells express tryptase, chymase, cathepsin G, and mast cell carboxypeptidase, while MC(T) cells express only tryptase. Both mast cell phenotypes were assessed near regions of osteolysis in uninfected failed joint implants by immunohistochemistry with antibodies to tryptase and chymase, and by in situ hybridization with anti-sense RNA probes for the respective mRNA molecules. Specimens from the interface membrane of 32 aseptically loosened total hip implants were studied, 28 of which had mast cells of the MC(TC) type. Most of these mast cells were aligned along the bone-prosthesis interface adjacent to loosened implants, suggesting involvement in the chronic inflammatory response that leads to bone resorption. Further ultrastructural evidence of mast cells in tissues from failed joint interface membranes was shown by transmission electron microscopy, and detection by staining after magnetic activated cell sorting. The presence of MC at the periprosthetic interface of eroded bone suggests MC degranulation and activity are associated with osteolysis in failed joint prostheses.
人类肥大细胞（MC）根据其分泌颗粒中的蛋白酶含量表现出两种不同的表型。MC(TC)细胞表达类胰蛋白酶、糜蛋白酶、组织蛋白酶G和肥大细胞羧肽酶，而MC(T)细胞仅表达类胰蛋白酶。通过使用抗类胰蛋白酶和抗糜蛋白酶抗体进行免疫组织化学，以及使用针对各自mRNA分子的反义RNA探针进行原位杂交，在未感染的失败关节植入物的骨溶解区域附近评估了两种肥大细胞表型。研究了32个无菌性松动全髋关节植入物界面膜的标本，其中28个有MC(TC)型肥大细胞。这些肥大细胞大多沿着与松动植入物相邻的骨-假体界面排列，表明其参与了导致骨吸收的慢性炎症反应。通过透射电子显微镜以及磁激活细胞分选后染色检测，进一步证明了失败关节界面膜组织中肥大细胞的超微结构证据。磨损骨假体周围界面处存在MC表明MC脱颗粒和活性与失败关节假体中的骨溶解有关。
