嗜热古菌火球菌OT3中核糖核酸酶P亚基间的蛋白质-蛋白质相互作用

Protein-protein interactions in the subunits of ribonuclease P in the hyperthermophilic archaeon Pyrococcus horikoshii OT3.

作者信息

Kifusa Mayumi, Fukuhara Hideo, Hayashi Takeshi, Kimura Makoto

机构信息

Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan.

出版信息

Biosci Biotechnol Biochem. 2005 Jun;69(6):1209-12. doi: 10.1271/bbb.69.1209.

DOI:10.1271/bbb.69.1209

PMID:15973057

Abstract

Ribonuclease P (RNase P) is a ribonucleoprotein complex involved in the processing of the 5' leader sequence of precursor tRNA (pre-tRNA). RNase P in the hyperthermophilic archaeon Pyrococcus horikoshii OT3 consists of RNA and five protein subunits (Ph1481p, Ph1496p, Ph1601p, Ph1771p, and Ph1877p). In vivo interactions among five protein subunits of RNase P in P. horikoshii OT3 were examined using a yeast two-hybrid system. The analysis indicates that proteins Ph1481p and Ph1601p interact strongly with Ph1877p and Ph1771p respectively, whereas Ph1481p interacts moderately with Ph1601p. In contrast, no interaction was detected between Ph1496p and the other four proteins. Co-immunoprecipitation analysis confirmed the interactions obtained by yeast two-hybrid assay.

摘要

核糖核酸酶P（RNase P）是一种核糖核蛋白复合体，参与前体tRNA（pre-tRNA）5'前导序列的加工过程。嗜热古菌火之神栖热袍菌OT3中的RNase P由RNA和五个蛋白质亚基（Ph1481p、Ph1496p、Ph1601p、Ph1771p和Ph1877p）组成。利用酵母双杂交系统检测了火之神栖热袍菌OT3中RNase P的五个蛋白质亚基之间的体内相互作用。分析表明，蛋白质Ph1481p和Ph1601p分别与Ph1877p和Ph1771p强烈相互作用，而Ph1481p与Ph1601p中度相互作用。相比之下，未检测到Ph1496p与其他四种蛋白质之间的相互作用。免疫共沉淀分析证实了酵母双杂交试验获得的相互作用。