Ultrahigh density microarrays of solid samples.

作者信息

LeBaron Matthew J, Crismon Heidi R, Utama Fransiscus E, Neilson Lynn M, Sultan Ahmed S, Johnson Kevin J, Andersson Eva C, Rui Hallgeir

机构信息

Lombardi Comprehensive Cancer Center, Department of Oncology, Georgetown University Medical Center, NRB E504, 3970 Reservoir Rd. NW, Washington, DC 20057-1469, USA.

出版信息

Nat Methods. 2005 Jul;2(7):511-3. doi: 10.1038/nmeth772.

DOI:10.1038/nmeth772

PMID:15973421

Abstract

We present a sectioning and bonding technology to make ultrahigh density microarrays of solid samples, cutting edge matrix assembly (CEMA). Maximized array density is achieved by a scaffold-free, self-supporting construction with rectangular array features that are incrementally scalable. This platform technology facilitates arrays of >10,000 tissue features on a standard glass slide, inclusion of unique sample identifiers for improved manual or automated tracking, and oriented arraying of stratified or polarized samples.

摘要