Zhang Heng-hui, Yang Hui-xia, Xi Hong-li, He Yu, Piao Wen-hua, Zhao Hong, Yu Min, Wang Gui-qiang
First Hospital of Peking University, Beijing l00034, China.
Zhonghua Gan Zang Bing Za Zhi. 2005 Jun;13(6):417-20.
To investigate the phenotypes and functions of cord blood dendritic cells of fetuses whose mothers are patients with chronic hepatitis B.
Peripheral blood and cord blood mononuclear cells (PBMC) were isolated from whole blood by density gradient centrifugation with Ficoll-Hypaque. The adherent cells were cultured in AIM-V medium containing recombinant human IL-4, TNF-alpha and GM-CSF. On day 9, mature DCs (mDC) were harvested and used for phenotype analysis. The amounts of IL-12 which dendritic cells produced were measured. The dendritic cells that were studied and compared were from cord blood of fetuses of both CHB positive and negative mothers and from CHC adult peripheral blood.
The expression rate of CD80 and CD83 of chronic hepatitis B mother cord blood dendritic cells was low compared with that of the healthy cord blood, healthy adult peripheral blood, and chronic hepatitis B adult peripheral blood, P < 0.05. The amount of IL-12 produced by chronic hepatitis B mother cord blood dendritic cells was lower than that of healthy cord blood, healthy adult peripheral blood, chronic hepatitis B adult peripheral blood (P < 0.05). The T lymphocyte proliferation inducing ability of dendritic cells of healthy adult peripheral blood was higher in inducing cord blood T lymphocytes proliferation, which was greater than that of the healthy adult peripheral blood in inducing adult T lymphocytes and was greater than that of the healthy cord blood dendritic cells in inducing cord blood T lymphocytes, which was greater than that of the healthy cord blood in inducing adult T lymphocytes, which was greater than that of chronic hepatitis B mothers in inducing cord blood T lymphocytes, which was greater than that of chronic hepatitis B mother cord blood in inducing adult T lymphocytes.
The maturation and functioning of CHB mother cord blood dendritic cells were lower than those of healthy cord blood, healthy adult peripheral blood and CHB adult peripheral blood.
研究母亲为慢性乙型肝炎患者的胎儿脐带血树突状细胞的表型和功能。
采用Ficoll-Hypaque密度梯度离心法从全血中分离外周血和脐带血单个核细胞（PBMC）。将贴壁细胞培养于含重组人白细胞介素-4、肿瘤坏死因子-α和粒细胞巨噬细胞集落刺激因子的AIM-V培养基中。第9天收获成熟树突状细胞（mDC）用于表型分析。检测树突状细胞产生的白细胞介素-12的量。所研究和比较的树突状细胞来自慢性乙型肝炎阳性和阴性母亲的胎儿脐带血以及慢性丙型肝炎成年外周血。
慢性乙型肝炎母亲脐带血树突状细胞CD80和CD83的表达率低于健康脐带血、健康成人外周血和慢性乙型肝炎成年外周血，P<0.05。慢性乙型肝炎母亲脐带血树突状细胞产生的白细胞介素-12的量低于健康脐带血、健康成人外周血、慢性乙型肝炎成年外周血（P<0.05）。健康成人外周血树突状细胞诱导脐带血T淋巴细胞增殖的能力较高，大于其诱导成人T淋巴细胞的能力，大于健康脐带血树突状细胞诱导脐带血T淋巴细胞的能力，大于健康脐带血诱导成人T淋巴细胞的能力，大于慢性乙型肝炎母亲诱导脐带血T淋巴细胞的能力，大于慢性乙型肝炎母亲脐带血诱导成人T淋巴细胞的能力。
慢性乙型肝炎母亲脐带血树突状细胞的成熟度和功能低于健康脐带血、健康成人外周血和慢性乙型肝炎成年外周血。
