Gao Jian, Chen Min, Ren Hong
Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Zhonghua Gan Zang Bing Za Zhi. 2005 Jun;13(6):432-5.
To investigate clinical effects of dendritic cells (DCs) pulsed with autologous hepatoma cell lysates on postoperative recurrence and metastasis of hepatocellular carcinoma (HCC).
DCs isolated from peripheral blood mononuclear cells of HCC patients were cultured and propagated in vitro using rhGM-CSF and rhIL-4, and then were pulsed with autologous hepatoma cell lysates. Thirty postoperative patients with HCC were randomly divided into two groups. Fifteen cases were treated with DC vaccine; fifteen cases received chemotherapy only as a control group. Immune function, clinical effects, hepatic tumor recurrence rate and the survival rate of patients of the two groups were observed and compared.
The levels of CD3+, CD4+/CD8+ and NK cells in the DC vaccine group were significantly increased after vaccination, while those of the control group had no significant changes. The concentration of IL-10 in the DC vaccine group was significantly decreased after the vaccination (P < 0.05). The hepatic tumor recurrence rate at 18 months in the DC vaccine group was 13.33%, compared with 53.33% in the control group (P < 0.05). The survival rate in the former was 93.33%, compared with that of 60% in the later (P < 0.05).
Hepatoma cell lysates pulsed DC vaccine may improve the immune function of the postoperative HCC patients and play an important role in prevention of postoperative recurrence and metastasis of HCC, which would provide an innovative approach for the immunotherapy of HCC.
探讨自体肝癌细胞裂解物脉冲负载的树突状细胞（DCs）对肝细胞癌（HCC）术后复发和转移的临床疗效。
从HCC患者外周血单个核细胞中分离DCs，体外使用重组人粒细胞巨噬细胞集落刺激因子（rhGM-CSF）和重组人白细胞介素-4（rhIL-4）进行培养和扩增，然后用自体肝癌细胞裂解物脉冲负载。30例HCC术后患者随机分为两组。15例接受DC疫苗治疗；15例仅接受化疗作为对照组。观察并比较两组患者的免疫功能、临床疗效、肝肿瘤复发率和生存率。
DC疫苗组接种后CD3⁺、CD4⁺/CD8⁺和自然杀伤细胞（NK细胞）水平显著升高，而对照组无明显变化。DC疫苗组接种后白细胞介素-10（IL-10）浓度显著降低（P<0.05）。DC疫苗组18个月时肝肿瘤复发率为13.33%，对照组为53.33%（P<0.05）。前者生存率为93.33%，后者为60%（P<0.05）。
肝癌细胞裂解物脉冲负载的DC疫苗可提高HCC术后患者的免疫功能，对预防HCC术后复发和转移起重要作用，为HCC免疫治疗提供了一种创新方法。
