Wang Kai-feng, Ye Sheng-long, Xue Qiong, Song Li-jie, Tian Bo, Liang Chun-min, Weng Yong-qiang, Tang Zhao-you
Liver Cancer Institute of Fudan University, Zhongshan Hospital, Shanghai 200032, China.
Zhonghua Gan Zang Bing Za Zhi. 2005 Jun;13(6):443-6.
To investigate the effectiveness of reconstruction of immunological functions of T cells on the degree of metastases of mouse hepatocarcinoma and the mechanisms of their functioning.
The T cell model of immunological functions in Balb/c nu/nu mice was established and the effectiveness of the model was evaluated. The mice were divided into 4 groups. The immunological functions of T cells in experiment groups of Balb/c nu/nu mice were reconstructed. Metastases of the cancer in lymph nodes in each group were examined histologically. The formation time and growth rate of the tumors were calculated. The expression of MHCI and II of the tumor cell line and the difference of expression of immune associated gene were detected by Th1-Th2-Th3 gene array.
The ratio of CD3, CD4, CD8 and CD4/CD8 in the reconstructed group was higher than that in the control group. The average formation time was 7.7+/-0.6 days in Balb/c nu/nu mice and 11.5+/-1.3 days in Balb/c mice. The extent of metastases of the experiment group was lower than that of the control group (P < 0.05). The expression of MHCI of the high metastasis cell line was lower than that of the low metastasis cell line (P < 0.05). The expressions of Th1/Th2 associated genes in lymphocytes of high metastasis mice were lower than those of the low metastasis mice.
Reconstruction of the immunological function of T cells can influence the metastasis of mouse hepatocarcinoma. The alteration of MHC molecule and low expression of Th1/Th2 correlated genes in lymphocytes may be a factor influencing the metastasis of liver cancer.
探讨T细胞免疫功能重建对小鼠肝癌转移程度的影响及其作用机制。
建立Balb/c裸鼠T细胞免疫功能模型并评估其有效性。将小鼠分为4组，对Balb/c裸鼠实验组的T细胞免疫功能进行重建。对每组小鼠淋巴结中的癌转移情况进行组织学检查，计算肿瘤的形成时间和生长速率。通过Th1-Th2-Th3基因芯片检测肿瘤细胞系MHCⅠ和Ⅱ的表达以及免疫相关基因表达的差异。
重建组中CD3、CD4、CD8及CD4/CD8的比例高于对照组。Balb/c裸鼠的平均形成时间为7.7±0.6天，Balb/c小鼠为11.5±1.3天。实验组的转移程度低于对照组（P<0.05）。高转移细胞系的MHCⅠ表达低于低转移细胞系（P<0.05）。高转移小鼠淋巴细胞中Th1/Th2相关基因的表达低于低转移小鼠。
T细胞免疫功能重建可影响小鼠肝癌的转移。MHC分子的改变及淋巴细胞中Th1/Th2相关基因的低表达可能是影响肝癌转移的一个因素。
