Zhang Enming, Li Xiaolong, Zhang Shufang, Chen Liangqiang, Zheng Xiaoxiang
Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
Biochem Biophys Res Commun. 2005 Aug 12;333(4):1171-7. doi: 10.1016/j.bbrc.2005.05.200.
Research on stem-cell transplantation has indicated that the success of transplantation largely depends on synchronizing donor cells into the G0/G1 phase. In this study, we investigated the profile of embryonic stem (ES) cell synchronization and its effect on the formation of embryonic bodies (EBs) using cell culture with serum deprivation. The D3 cell line of ES cells was used, and parameters such as cell proliferation and activity, EB formation, and expression of stage-specific embryonic antigen-1 and Oct-4 were investigated. Results showed that the percentage of G0/G1 stage in serum deprivation culture is significantly higher than that in culture with serum supplementation. Synchronized ES cells can reenter the normal cell cycle successfully after serum supply. EBs formed from synchronized ES cells have higher totipotency capability to differentiate into functional neuronal cells than EBs formed from unsynchronized ES cells. Our study provides a method for ES treatment before cell transplantation that possibly helps to decrease the rate of cell death after transplantation.
干细胞移植研究表明，移植的成功很大程度上取决于将供体细胞同步到G0/G1期。在本研究中，我们使用血清剥夺细胞培养法，研究了胚胎干细胞（ES细胞）同步化的情况及其对胚体（EBs）形成的影响。使用了ES细胞的D3细胞系，并研究了细胞增殖和活性、EB形成以及阶段特异性胚胎抗原-1和Oct-4的表达等参数。结果表明，血清剥夺培养中G0/G1期的百分比显著高于血清补充培养中的百分比。同步化的ES细胞在补充血清后能够成功重新进入正常细胞周期。与未同步化的ES细胞形成的EBs相比，由同步化的ES细胞形成的EBs具有更高的全能性，能够分化为功能性神经元细胞。我们的研究提供了一种在细胞移植前进行ES治疗的方法，这可能有助于降低移植后细胞死亡的发生率。
