Rai R, Richardson C, Flecknell P, Robertson H, Burt A, Manas D M
Department of Surgery, Russell's Hall Hospital, Dudley, UK.
J Surg Res. 2005 Nov;129(1):147-51. doi: 10.1016/j.jss.2005.03.020. Epub 2005 Jun 22.
Radiofrequency ablation (RFA) of the liver produces necrosis of the hepatocytes. Histological examination shows a sharp demarcation between ablated and normal liver tissue. This experiment was carried out to study the cellular injury produced by RFA in area surrounding the ablated tissue and effect of reperfusion on this zone.
Five pigs underwent RFA of liver parenchyma. Four pigs were sacrificed 30 min after RFA and one pig was sacrificed 5 days later. Ablated lesions including surrounding liver parenchyma was examined for apoptosis and HSP 70 expression.
There was a zone of transition surrounding the necrotic ablated area that showed apoptosis as well as increased HSP 70 expression. This was more prevalent in the pig that was sacrificed 5 days later.
RFA produces sub lethal injury in the zone of transition causing apoptosis and increase in HSP 70 expression. Increased HSP expression enhances immunogenicity of these cells that can have therapeutic implications for the treatment of liver.
肝脏射频消融（RFA）会导致肝细胞坏死。组织学检查显示消融组织与正常肝组织之间有明显界限。进行本实验以研究RFA在消融组织周围区域产生的细胞损伤以及再灌注对该区域的影响。
对5头猪的肝实质进行RFA。4头猪在RFA后30分钟处死，1头猪在5天后处死。检查包括周围肝实质的消融病灶的凋亡情况和热休克蛋白70（HSP 70）表达。
在坏死消融区域周围有一个过渡带，显示出凋亡以及HSP 70表达增加。这在5天后处死的猪中更为普遍。
RFA在过渡带产生亚致死性损伤，导致凋亡并使HSP 70表达增加。HSP表达增加增强了这些细胞的免疫原性，这可能对肝脏治疗具有重要意义。
