Boselli Emmanuel, Guillier Marion, Freney Jean, Mazoyer Marie-Andrée, Casoli Emmanuelle, Renaud François R N, Rimmelé Thomas, Chassard Dominique, Allaouchiche Bernard
Service d'Anesthésie-Réanimation, Department of Anesthesiology, Hôpital Edouard Herriot, 5 place d'Arsonval, 69437 Lyon cedex 03, France.
Anesth Analg. 2005 Jul;101(1):121-4, table of contents. doi: 10.1213/01.ANE.0000153500.68193.F7.
We conducted an in vitro study to investigate the antibacterial activity of clonidine and neostigmine on common microorganisms encountered during infectious complications after regional anesthesia. Standardized suspensions of Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli were incubated during 1, 3, 6, and 24 h at 37 degrees C with concentrations of 37.5, 75, and 150 microg/mL of clonidine and 125, 250, and 500 microg/mL of neostigmine. After 24 h incubation at 37 degrees C, the colony counts were compared by two-way analysis of variance. The mean colony counts for S. aureus decreased significantly from control as the exposure to clonidine increased (P < 0.05), with a approximately 100% kill at 6 h for the largest concentration (150 microg/mL) and at 24 h for the intermediate concentration (75 microg/mL). Similar results were observed for S. epidermidis, with a approximately 100% kill at 6 h for the largest concentrations (75 and 150 microg/mL). No bactericidal activity of clonidine was observed for E. coli and no bactericidal activity of neostigmine was observed for any of the tested strains. In the conditions of this experiment, clonidine, but not neostigmine, exhibited a concentration-dependent and time-dependent bactericidal activity in vitro on the microorganisms most frequently encountered in infectious complications after regional anesthesia.
我们进行了一项体外研究，以调查可乐定和新斯的明对区域麻醉后感染并发症中常见微生物的抗菌活性。将金黄色葡萄球菌、表皮葡萄球菌和大肠杆菌的标准化悬液在37℃下分别与浓度为37.5、75和150μg/mL的可乐定以及125、250和500μg/mL的新斯的明孵育1、3、6和24小时。在37℃孵育24小时后，通过双向方差分析比较菌落计数。随着可乐定暴露量增加，金黄色葡萄球菌的平均菌落计数与对照组相比显著降低（P<0.05），最大浓度（150μg/mL）在6小时时杀灭率约为100%，中间浓度（75μg/mL）在24小时时杀灭率约为100%。表皮葡萄球菌也观察到类似结果，最大浓度（75和150μg/mL）在6小时时杀灭率约为100%。未观察到可乐定对大肠杆菌有杀菌活性，也未观察到新斯的明对任何测试菌株有杀菌活性。在本实验条件下，可乐定而非新斯的明在体外对区域麻醉后感染并发症中最常见的微生物表现出浓度依赖性和时间依赖性杀菌活性。
