Ishibashi Tomoko, Usami Takeshi, Fujie Manabu, Azumi Kaoru, Satoh Nori, Fujiwara Shigeki
Department of Materials Science, Faculty of Science, Kochi University, Kochi, Japan.
Dev Dyn. 2005 Aug;233(4):1571-8. doi: 10.1002/dvdy.20486.
Oligonucleotide-based microarray analyses were carried out to identify retinoic acid target genes in embryos of the ascidian Ciona intestinalis. Of 21,938 spots, 50 (corresponding to 43 genes) showed over twofold up-regulation in retinoic acid-treated tail bud embryos. In situ hybridization verified retinoic acid-induced up-regulation of 23 genes. Many of them were expressed in the anterior tail region, where a retinaldehyde dehydrogenase homolog is expressed. Homologs of vertebrate genes involved in neurogenesis and/or neuronal functions (e.g., COUP-TF, Ci-Hox1, and SCO-spondin) were expressed in the central nervous system of Ciona embryos, and activated by retinoic acid. Genes encoding transcription factors (e.g., Ci-lmx1.2, vitamin D receptor, and Hox proteins) and apoptosis-related proteins (e.g., transglutaminase and an apoptosis-inducing factor homolog) were also activated by retinoic acid. Simultaneous treatment of embryos with retinoic acid and puromycin revealed a few direct targets, including genes encoding Ci-Hox1, Ci-Cyp26, and an Rnf126-like ring finger protein.
开展了基于寡核苷酸的微阵列分析，以鉴定海鞘（Ciona intestinalis）胚胎中的视黄酸靶基因。在21,938个斑点中，有50个（对应43个基因）在视黄酸处理的尾芽胚胎中显示出两倍以上的上调。原位杂交证实了视黄酸诱导的23个基因的上调。其中许多基因在前尾区域表达，视网膜醛脱氢酶同源物也在此区域表达。参与神经发生和/或神经元功能的脊椎动物基因的同源物（例如，COUP-TF、Ci-Hox1和SCO-spondin）在海鞘胚胎的中枢神经系统中表达，并被视黄酸激活。编码转录因子（例如，Ci-lmx1.2、维生素D受体和Hox蛋白）和凋亡相关蛋白（例如，转谷氨酰胺酶和凋亡诱导因子同源物）的基因也被视黄酸激活。用视黄酸和嘌呤霉素同时处理胚胎揭示了一些直接靶标，包括编码Ci-Hox1、Ci-Cyp26和Rnf126样环指蛋白的基因。
