Duan Zhong-ping, Zhao Xiu-ying, Huang De-zhuang, He Li-xiang, Chen Yu, Zhao Chun-hui, Zheng Bo-jian
Beijing You-An Hospital, Capital University of Medical Science, Beijing 100054, China.
Chin Med J (Engl). 2005 Jun 5;118(11):909-14.
Regulated on activation, normal T-cell expressed and secreted (RANTES) plays a critical role in T-lymphocyte activation and proliferation. The process is involved in both acute and chronic phases of inflammation. The present study was to ascertain the possible correlations between chronic hepatitis B virus (HBV) infection and the RANTES gene polymorphisms and their expression.
The study included 130 HBV negative healthy donors and 152 patients with chronic hepatitis B (CHB) virus infection. The polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLPs) were used to detect RANTES gene single nucleotide polymorphisms (SNPs). RANTES levels in the platelet depleted plasma were detected by enzyme linked immunosorbent assay (ELISA).
RANTES alleles -403G, -28C and In1.1T were the predominant alleles in the subjects studied. No significant correlation was found between CHB infection and the RANTES alleles, while a significant correlation was found between CHB infection and increased RANTES expression in platelet depleted plasma (P < 0.05).
SNPs in RANTES gene do not affect chronic HBV infection or the outcome of interferon-alpha treatment in patients positive for HBV "e" antigen (HBeAg+). However, patients with CHB infection express the higher levels of plasma RANTES, which is thus associated with CHB infection.
活化调节正常T细胞表达和分泌因子（RANTES）在T淋巴细胞活化和增殖中起关键作用。该过程涉及炎症的急性和慢性阶段。本研究旨在确定慢性乙型肝炎病毒（HBV）感染与RANTES基因多态性及其表达之间的可能相关性。
本研究纳入130名HBV阴性健康供者和152例慢性乙型肝炎（CHB）病毒感染患者。采用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）检测RANTES基因单核苷酸多态性（SNP）。采用酶联免疫吸附测定（ELISA）检测去血小板血浆中RANTES水平。
RANTES等位基因-403G、-28C和In1.1T是所研究对象中的主要等位基因。CHB感染与RANTES等位基因之间未发现显著相关性，而CHB感染与去血小板血浆中RANTES表达增加之间存在显著相关性（P<0.05）。
RANTES基因中的单核苷酸多态性不影响慢性HBV感染或乙肝“e”抗原（HBeAg+）阳性患者的α干扰素治疗结果。然而，CHB感染患者血浆RANTES表达水平较高，因此与CHB感染相关。
