Montagnani Caroline, Tirape Ana, Boulo Viviane, Escoubas Jean-Michel
Génome, Populations, Interactions, Adaptation (GPIA), UMR5171 (IFREMER, CNRS, UMII) Université de Montpellier II, place Eugène Bataillon, CC80, 34095 Montpellier, France.
Dev Comp Immunol. 2005;29(10):831-9. doi: 10.1016/j.dci.2005.02.005. Epub 2005 Mar 21.
We previously characterized a Crassostrea gigas tissue inhibitor of metalloproteinase (Cg-timp 1.3) with potential role in wound healing and defense mechanisms. Here we isolated a second cDNA (Cg-timp 1.1) encoding a protein that contains the characteristic signature of TIMP proteins. Sequence analysis of the two transcripts showed that they originate from two distinct genes. The two proteins, Cg-TIMP 1.1 and 1.3, are closely related and share 81% identity. Northern blot analysis of Cg-timp gene expression in adult oyster hemocytes indicated that the ratio between the two transcripts was constant from one oyster to another (Cg-timp 1.1 and 1.3 represent 32 and 68%, respectively). Conversely, during ontogenesis the expression pattern of the two Cg-timp genes was different. Indeed, Cg-timp 1.3 mRNAs were detected from the larval D stage whereas Cg-timp 1.1 transcripts were undetectable up to 22 days post-fertilization. The difference in expression pattern of the two Cg-timp genes may reveal distinct implications of these genes in the embryos and larvae developments.
我们之前鉴定了一种太平洋牡蛎金属蛋白酶组织抑制剂（Cg-timp 1.3），其在伤口愈合和防御机制中可能发挥作用。在此，我们分离出了第二个cDNA（Cg-timp 1.1），它编码一种含有TIMP蛋白特征性结构域的蛋白质。对这两种转录本的序列分析表明，它们源自两个不同的基因。这两种蛋白质，Cg-TIMP 1.1和1.3，亲缘关系密切，具有81%的同一性。对成年牡蛎血细胞中Cg-timp基因表达的Northern印迹分析表明，两种转录本之间的比例在不同牡蛎个体中保持恒定（Cg-timp 1.1和1.3分别占32%和68%）。相反，在个体发育过程中，两个Cg-timp基因的表达模式有所不同。实际上，在幼虫D期可检测到Cg-timp 1.3的mRNA，而在受精后22天之前均未检测到Cg-timp 1.1的转录本。两个Cg-timp基因表达模式的差异可能揭示了这些基因在胚胎和幼虫发育中的不同作用。
