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用于表征贴壁薄生物样品杨氏模量和泊松比的微吸管抽吸实验的扩展建模:数值和实验研究。

An extended modeling of the micropipette aspiration experiment for the characterization of the Young's modulus and Poisson's ratio of adherent thin biological samples: numerical and experimental studies.

作者信息

Boudou Thomas, Ohayon Jacques, Arntz Youri, Finet Gérard, Picart Catherine, Tracqui Philippe

机构信息

Laboratoire TIMC-IMAG, Equipe DynaCell, CNRS UMR 5525, Institut de l'Ingénierie et de l'Information de Santé, Faculté de Médecine de Grenoble, 38706 La Tronche Cedex, France.

出版信息

J Biomech. 2006;39(9):1677-85. doi: 10.1016/j.jbiomech.2005.04.026. Epub 2005 Jun 23.

Abstract

The micropipette aspiration (MA) experiment remains a quite widely used micromanipulation technique for quantifying the elastic modulus of cells and, less frequently, of other biological samples. However, moduli estimations derived from MA experiments are only valid if the probed sample is non-adherent to the rigid substrate. This study extends this standard formulation by taking into account the influence of the sample adhesion. Using a finite element analysis of the sample aspiration into the micropipette, we derived a new expression of the aspirated length for linear elastic materials. Our results establish that (i) below a critical value, the thickness h of the probed sample must be considered to get an accurate value of its Young's modulus (ii) this critical value depends both on the Poisson's ratio and on the sample adhesivity. Additionally, we propose a novel method which allows the computation of the intrinsic Young's modulus of the adherent probed sample from its measured apparent elasticity modulus. Thanks to the set of computational graphs we derived from our theoretical analysis, we successfully validate this method by experiments performed on polyacrylamide gels. Interestingly, the original procedure we proposed allows a simultaneous quantification of the Young's modulus and of the Poisson's ratio of the adherent gel. Thus, our revisited analysis of MA experiments extends the application domain of this technique, while contributing to decrease the dispersion of elastic modulus values obtained by this method.

摘要

微量吸管抽吸(MA)实验仍然是一种广泛使用的微操纵技术,用于量化细胞的弹性模量,较少用于量化其他生物样本的弹性模量。然而,只有在被探测样本不粘附于刚性基质时,从MA实验得出的模量估计才有效。本研究通过考虑样本粘附的影响扩展了这一标准公式。通过对样本被吸入微量吸管的有限元分析,我们推导出了线性弹性材料的吸入长度的新表达式。我们的结果表明:(i)在临界值以下,必须考虑被探测样本的厚度h才能获得其杨氏模量的准确值;(ii)该临界值取决于泊松比和样本粘附性。此外,我们提出了一种新方法,该方法可以根据测量的表观弹性模量计算粘附被探测样本的固有杨氏模量。借助我们从理论分析中得出的一组计算图,我们通过在聚丙烯酰胺凝胶上进行的实验成功验证了该方法。有趣的是,我们提出的原始程序可以同时量化粘附凝胶的杨氏模量和泊松比。因此,我们对MA实验的重新分析扩展了该技术的应用领域,同时有助于减少通过该方法获得的弹性模量值的离散度。

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