Center for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, Spain; Departamento de Ciencia de Materiales, ETSI de Caminos, Canales y Puertos, Universidad Politécnica de Madrid, Madrid, Spain.
Center for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, Spain; Departamento de Ciencia de Materiales, ETSI de Caminos, Canales y Puertos, Universidad Politécnica de Madrid, Madrid, Spain; Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain.
Biophys J. 2019 Feb 19;116(4):587-594. doi: 10.1016/j.bpj.2019.01.004. Epub 2019 Jan 7.
With five decades of sustained application, micropipette aspiration has enabled a wide range of biomechanical studies in the field of cell mechanics. Here, we provide an update on the use of the technique, with a focus on recent developments in the analysis of the experiments, innovative microaspiration-based approaches, and applications in a broad variety of cell types. We first recapitulate experimental variations of the technique. We then discuss analysis models focusing on important limitations of widely used biomechanical models, which underpin the urge to adopt the appropriate ones to avoid misleading conclusions. The possibilities of performing different studies on the same cell are also considered.
经过五十年的持续应用，微量移液器吸液技术已经在细胞力学领域实现了广泛的生物力学研究。在这里，我们提供了该技术的最新应用情况，重点介绍了实验分析方面的最新进展、基于微吸液的创新方法以及在各种细胞类型中的应用。我们首先总结了该技术的实验变化，然后讨论了分析模型，重点介绍了广泛使用的生物力学模型的重要局限性，这些局限性促使人们采用适当的模型来避免产生误导性结论。我们还考虑了在同一细胞上进行不同研究的可能性。
