Eko F O, Rotimi V O, Coker A O
Department of Medical Microbiology and Parasitology, College of Medicine of University of Lagos, Nigeria.
J Trop Med Hyg. 1992 Jun;95(3):202-5.
Clinical and environmental isolates of Vibrio parahaemolyticus were examined for their ability to agglutinate human and rabbit erythrocyte and to adhere to human buccal epithelial (HBE) cells in the presence or absence of mannose. All strains produced cell-associated haemagglutinins (HGs) after 3 h at 37 degrees C. Mannose-sensitive haemagglutination (MSHA) appeared to be a significant marker for differentiating between clinical and environmental isolates; 75% of clinical and 11% of environmental isolates exhibited MSHA with rabbit erythrocytes. All strains showed mannose-resistant adhesion (MRA) to HBE cells whose pattern had no relationship to the epidemiological source of the isolates. Adherence to HBE cells correlated with haemagglutination (HA) capability of environmental but not clinical isolates. This suggests that although intestinal adherence may be an essential step in vibrio colonization, it may not be a sufficient prerequisite for the subsequent expression of pathogenicity in Vibrio parahaemolyticus.
对副溶血性弧菌的临床分离株和环境分离株进行了检测，以观察它们在有无甘露糖的情况下凝集人及兔红细胞以及黏附人颊黏膜上皮（HBE）细胞的能力。所有菌株在37℃培养3小时后均产生细胞相关血凝素（HGs）。甘露糖敏感血凝反应（MSHA）似乎是区分临床分离株和环境分离株的一个重要标志；75%的临床分离株和11%的环境分离株对兔红细胞表现出MSHA。所有菌株均显示对HBE细胞有甘露糖抗性黏附（MRA），其模式与分离株的流行病学来源无关。对HBE细胞的黏附与环境分离株而非临床分离株的血凝反应（HA）能力相关。这表明，虽然肠道黏附可能是弧菌定植的一个必要步骤，但它可能不是副溶血性弧菌随后表达致病性的充分前提条件。
