Parra-Delgado Hortensia, García-Pillado Fátima, Sordo Monserrat, Ramírez-Apan Teresa, Martínez-Vázquez Mariano, Ostrosky-Wegman Patricia
Instituto de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Circuito Exterior, Coyoacán, C. P. 04510, México, D.F.
Life Sci. 2005 Oct 14;77(22):2855-65. doi: 10.1016/j.lfs.2005.05.024.
Argentatins A and B are abundant triterpenes present in Parthenium argentatum. Both compounds have shown cytotoxic properties on K562, MCF-7, PC-3, HCT-15 and U251 human cancer cell lines. Furthermore the cytotoxic, cytostatic and genotoxic effects of the argentatins on proliferating lymphocytes were evaluated using cytokinesis-block micronucleus test. Argentatin A had no cytostatic properties, but it was cytotoxic for proliferating lymphocytes at a concentration of 25 microM (P < 0.005). On the other hand, argentatin B showed significant cytostatic effects (P < 0.001) at concentrations of 5 to 25 microM and it did not show cytotoxic effects at the same concentrations. Neither argentatin showed genotoxic effects in terms of micronucleus frequency in human lymphocytes. According to these results the argentatins are not able to cause injury on DNA by clastogenic or aneugenic mechanisms.
银胶菊素A和B是银胶菊中含量丰富的三萜类化合物。这两种化合物对K562、MCF - 7、PC - 3、HCT - 15和U251人癌细胞系均表现出细胞毒性。此外，使用胞质分裂阻滞微核试验评估了银胶菊素对增殖淋巴细胞的细胞毒性、细胞生长抑制和遗传毒性作用。银胶菊素A没有细胞生长抑制特性，但在浓度为25微摩尔时对增殖淋巴细胞具有细胞毒性（P < 0.005）。另一方面，银胶菊素B在浓度为5至25微摩尔时表现出显著的细胞生长抑制作用（P < 0.001），且在相同浓度下未表现出细胞毒性作用。就人淋巴细胞中的微核频率而言，两种银胶菊素均未表现出遗传毒性作用。根据这些结果，银胶菊素不能通过断裂剂或非整倍体机制对DNA造成损伤。
