Roopesh Krishnan, Ramachandran Sumitra, Nampoothiri K Madhavan, Szakacs George, Pandey Ashok
Biotechnology Division, Regional Research Laboratory, CSIR, Trivandrum 695 019, India.
Bioresour Technol. 2006 Feb;97(3):506-11. doi: 10.1016/j.biortech.2005.02.046. Epub 2005 Apr 20.
Comparisons were made for phytase production using wheat bran (WB) and oilcakes as substrates in solid-state fermentation (SSF) by Mucor racemosus NRRL 1994. WB was also used as mixed substrate with oil cakes. Sesame oil cake (SOC) served as the best carbon source for phytase synthesis by the fungal strain as it gave the highest enzyme titres (30.6 U/gds). Groundnut oil cake (GOC) also produced a reasonably good quantity of enzyme (24.3 U/gds). Enzyme production on WB was surprisingly much less (almost 3.5 times less in comparison to SOC). Mixing WB with SOC (1:1 ratio) resulted in better phytase activity (32.2 U/gds). Optimization of various process parameters such as incubation time, initial moisture content and inoculum concentration was carried out using the single variable mode optimization technique. Under optimized conditions, the production of phytase reached 44.5 U/gds, which was almost 1.5-fold higher than the highest yield obtained with any individual substrate used in this study and was more than 4-fold higher than that obtained from WB.
用总状毛霉NRRL 1994在固态发酵（SSF）中以麦麸（WB）和油饼为底物生产植酸酶进行了比较。WB也与油饼作为混合底物使用。芝麻油饼（SOC）是该真菌菌株合成植酸酶的最佳碳源，因为它产生的酶活最高（30.6 U/gds）。花生油饼（GOC）也产生了相当数量的酶（24.3 U/gds）。在WB上的酶产量出奇地低得多（与SOC相比几乎少3.5倍）。将WB与SOC以1:1比例混合可产生更好的植酸酶活性（32.2 U/gds）。使用单变量模式优化技术对各种工艺参数如培养时间、初始水分含量和接种物浓度进行了优化。在优化条件下，植酸酶产量达到44.5 U/gds，这几乎比本研究中使用的任何单一底物获得的最高产量高1.5倍，并且比从WB获得的产量高4倍多。
