Dept. of Biochemistry, C.B.S.H, Govind Ballabh Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India.
Biotechnol Prog. 2012 Nov-Dec;28(6):1432-42. doi: 10.1002/btpr.1622. Epub 2012 Oct 18.
This article deals with the optimization of the various parameters for production of phytase using Achromobacter sp. PB-01 in submerged fermentation (SmF). A semisynthetic medium containing ingredients of phytase screening media (PSM) supplemented with 2% (w/v) sucrose, 1% (w/v) peptone, and 10% (w/v) wheat bran was found to be the best production medium among the various combinations tried. Among various surfactants added to SmF, Triton X-100 (0.1%) exhibited a 16% increase in phytase activity. An overall 11.2 fold enhancement in enzyme activity (0.79 U/mL→8.84 U/mL) was attained when SmF was carried out using 0.5% (v/v) inoculum of a 15 h old culture of Achromobacter sp. PB-01 at an initial pH of 5.5, temperature 30°C and allowed to grow for 48 h. Presence of accessory hydrolytic enzymes in the crude extract further added value as feed additive by mediating efficient degradation of non-starch polysaccharides (NSP). In addition, we also investigated the efficacy of phytase on different agro-industrial residues using in vitro experiments that simulated the conditions of the digestive tract. Results indicate that phytase from our source hydrolyze phytate efficiently with the concomitant liberation of inorganic phosphate, protein, reducing sugar, and calcium.
本文探讨了在深层发酵（SmF）中利用不动杆菌（Achromobacter sp.）PB-01 生产植酸酶时各种参数的优化。在各种尝试的组合中，发现含有植酸酶筛选培养基（PSM）成分的半合成培养基，添加 2%（w/v）蔗糖、1%（w/v）蛋白胨和 10%（w/v）麦麸，是最佳的生产培养基。在添加到 SmF 的各种表面活性剂中，Triton X-100（0.1%）使植酸酶活性增加了 16%。当使用 0.5%（v/v）接种物接种 15 小时龄的不动杆菌（Achromobacter sp.）PB-01 培养物，初始 pH 值为 5.5、温度为 30°C，并允许生长 48 小时时，SmF 的酶活性提高了 11.2 倍（0.79 U/mL→8.84 U/mL）。粗提物中存在辅助水解酶作为饲料添加剂进一步增加了价值，通过介导非淀粉多糖（NSP）的有效降解。此外，我们还使用模拟消化道条件的体外实验研究了植酸酶对不同农业工业残渣的功效。结果表明，我们来源的植酸酶可以有效地水解植酸盐，同时释放无机磷、蛋白质、还原糖和钙。
