Gu Xingxing, Xu Fang, Wang Xiaolin, Gao Xiang, Zhao Qingshun
Model Animal Research Center and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, China.
Gene Expr Patterns. 2005 Aug;5(6):733-9. doi: 10.1016/j.modgep.2005.04.005.
Proper restriction of retinoid signaling by Cyp26s is essential for development of vertebrate embryos while inappropriate retinoid signaling can cause teratogenesis. Here, we report cloning and expression analysis of a novel cyp26 gene (cyp26d1) isolated from zebrafish. The predicted protein encoded by cyp26d1 consists of 554 amino acids. It exhibits 54% amino acid identity with human Cyp26C1, 50% with zebrafish Cyp26B1 and 38% with zebrafish Cyp26A1. Whole-mount in situ hybridization shows that cyp26d1 is first expressed in sphere stage, then disappears at 50% epiboly and resumes its expression at 75% epiboly. During segmentation period, cyp26d1 message is found at presumptive hindbrain. Double in situ hybridization with krox20 and cyp26d1 reveals that cyp26d1 is expressed in presumptive rhombomere 2-4 (r2-r4) at 2-somite stage. At 3-somite stage, cyp26d1 gene is expressed in r6 and pharyngeal arch (pa) one in addition to its expression at r2 and r4. At 6-somite stage, cyp26d1 message is present in continuous bands at r2-r6 and in pa1. This expression pattern is maintained from 10-somite stage through 21-somite stage except that the expression level is greatly reduced at r2 and r4. At 21-somite stage, cyp26d1 is also found in a group of cells in telencephalon and diencephalons. At 25-31h post-fertilization (hpf), the zebrafish cyp26d1 expression domain is extended to eyes, otic vesicles and midbrain in addition to its expression in hindbrain, telencephalon, diencephalons, and pharyngeal arches. At 35-48hpf, the expression of cyp26d1 is mainly restricted to otic vesicles, pharyngeal arches and pectoral fins and the expression level is greatly reduced.
Cyp26s 对类视黄醇信号传导的适当限制对于脊椎动物胚胎的发育至关重要，而不适当的类视黄醇信号传导会导致致畸作用。在这里，我们报告了从斑马鱼中分离出的一种新型 cyp26 基因（cyp26d1）的克隆和表达分析。cyp26d1 编码的预测蛋白质由 554 个氨基酸组成。它与人类 Cyp26C1 的氨基酸同一性为 54%，与斑马鱼 Cyp26B1 的同一性为 50%，与斑马鱼 Cyp26A1 的同一性为 38%。整体原位杂交显示，cyp26d1 首先在囊胚期表达，然后在原肠胚形成 50% 时消失，并在原肠胚形成 75% 时恢复表达。在体节期，在假定的后脑发现 cyp26d1 信息。与 krox20 和 cyp26d1 的双重原位杂交显示，cyp26d1 在 2 体节期在假定的菱脑节 2-4（r2-r4）中表达。在 3 体节期，cyp26d1 基因除了在 r2 和 r4 表达外，还在 r6 和第一咽弓（pa）中表达。在 6 体节期，cyp26d1 信息存在于 r
