Watson Peter, Stephens David J
Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK.
Biochim Biophys Acta. 2005 Jul 10;1744(3):304-15. doi: 10.1016/j.bbamcr.2005.03.003. Epub 2005 Mar 23.
The transport of proteins and lipids between the endoplasmic reticulum and Golgi apparatus is initiated by the collection of secretory cargo from within the lumen of the endoplasmic reticulum. Subsequently, transport carriers are formed that bud from this membrane and are transported to, and subsequently merge with, the Golgi. The principle driving force behind the budding process is the multi-subunit coat protein complex, COPII. A considerable amount of information is now available regarding the molecular mechanisms by which COPII components operate together to drive cargo selection and transport carrier formation. In contrast, the precise nature of the transport carriers formed is still a matter of considerable debate. Vesicular and tubular carriers have been characterized that are, or in other cases are not, coated with the COPII complex. Here, we seek to integrate much of the data surrounding this topic and try to understand the mechanisms by which vesicular and/or tubular carriers might be generated.
蛋白质和脂质在内质网与高尔基体之间的运输始于从内质网腔中收集分泌性货物。随后，形成运输载体，这些载体从该膜上出芽并被运输到高尔基体，随后与高尔基体融合。出芽过程背后的主要驱动力是多亚基包被蛋白复合物COPII。目前已有大量关于COPII组分共同作用以驱动货物选择和运输载体形成的分子机制的信息。相比之下，所形成的运输载体的确切性质仍是一个备受争议的问题。已鉴定出有被或未被COPII复合物包被的囊泡状和管状载体。在这里，我们试图整合围绕这一主题的大量数据，并尝试理解可能产生囊泡状和/或管状载体的机制。
