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1
Connexin43 with a cytoplasmic loop deletion inhibits the function of several connexins.具有细胞质环缺失的连接蛋白43会抑制多种连接蛋白的功能。
Biochem Biophys Res Commun. 2005 Aug 12;333(4):1185-93. doi: 10.1016/j.bbrc.2005.05.201.
2
Connexin43 and connexin45 form heteromeric gap junction channels in which individual components determine permeability and regulation.连接蛋白43和连接蛋白45形成异源间隙连接通道，其中各个组分决定通透性和调节作用。
Circ Res. 2002 May 31;90(10):1100-7. doi: 10.1161/01.res.0000019580.64013.31.
3
Intracellular domains of mouse connexin26 and -30 affect diffusional and electrical properties of gap junction channels.小鼠连接蛋白26和30的细胞内结构域影响间隙连接通道的扩散和电学特性。
J Membr Biol. 2001 May 15;181(2):137-48. doi: 10.1007/s00232-001-0017-1.
4
N-terminal residues in Cx43 and Cx40 determine physiological properties of gap junction channels, but do not influence heteromeric assembly with each other or with Cx26.Cx43和Cx40中的N端残基决定了间隙连接通道的生理特性，但不影响它们彼此之间或与Cx26的异聚体组装。
J Cell Sci. 2006 Jun 1;119(Pt 11):2258-68. doi: 10.1242/jcs.02953.
5
Different domains are critical for oligomerization compatibility of different connexins.不同的结构域对于不同连接蛋白的寡聚体兼容性至关重要。
Biochem J. 2011 May 15;436(1):35-43. doi: 10.1042/BJ20110008.
6
Connexin43 and connexin26 form gap junctions, but not heteromeric channels in co-expressing cells.连接蛋白43和连接蛋白26形成间隙连接，但在共表达细胞中不形成异聚通道。
J Cell Sci. 2004 May 15;117(Pt 12):2469-80. doi: 10.1242/jcs.01084. Epub 2004 May 5.
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Heteromeric mixing of connexins: compatibility of partners and functional consequences.连接蛋白的异源混合：伙伴兼容性及功能后果
Cell Commun Adhes. 2001;8(4-6):199-204. doi: 10.3109/15419060109080723.
8
Specific permeability and selective formation of gap junction channels in connexin-transfected HeLa cells.连接蛋白转染的HeLa细胞中缝隙连接通道的特定通透性和选择性形成
J Cell Biol. 1995 May;129(3):805-17. doi: 10.1083/jcb.129.3.805.
9
A carboxyl terminal domain of connexin43 is critical for gap junction plaque formation but not for homo- or hetero-oligomerization.连接蛋白43的羧基末端结构域对于间隙连接斑的形成至关重要，但对于同型或异型寡聚化并非如此。
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Gap junction permeability: selectivity for anionic and cationic probes.缝隙连接通透性：对阴离子和阳离子探针的选择性。
Am J Physiol Cell Physiol. 2011 Mar;300(3):C600-9. doi: 10.1152/ajpcell.00316.2010. Epub 2010 Dec 9.

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GJA1 depletion causes ciliary defects by affecting Rab11 trafficking to the ciliary base.GJA1 缺失通过影响 Rab11 向纤毛基部的运输而导致纤毛缺陷。
Elife. 2022 Aug 25;11:e81016. doi: 10.7554/eLife.81016.
2
Connexin 43 channels are essential for normal bone structure and osteocyte viability.连接蛋白43通道对于正常的骨骼结构和骨细胞活力至关重要。
J Bone Miner Res. 2015 Mar;30(3):436-48. doi: 10.1002/jbmr.2374.
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Regulation of cellular communication by signaling microdomains in the blood vessel wall.血管壁中信号微区对细胞通讯的调节。
Pharmacol Rev. 2014 Mar 26;66(2):513-69. doi: 10.1124/pr.112.007351. Print 2014.
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Different domains are critical for oligomerization compatibility of different connexins.不同的结构域对于不同连接蛋白的寡聚体兼容性至关重要。
Biochem J. 2011 May 15;436(1):35-43. doi: 10.1042/BJ20110008.
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Connexin43 hemichannels contribute to cadmium-induced oxidative stress and cell injury.缝隙连接蛋白 43 半通道促进镉诱导的氧化应激和细胞损伤。
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Cx30.2 can form heteromeric gap junction channels with other cardiac connexins.Cx30.2可与其他心脏连接蛋白形成异聚体间隙连接通道。
Biochem Biophys Res Commun. 2008 May 2;369(2):388-94. doi: 10.1016/j.bbrc.2008.02.040. Epub 2008 Feb 20.
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Connexin43 increases the sensitivity of prostate cancer cells to TNFalpha-induced apoptosis.连接蛋白43增加前列腺癌细胞对肿瘤坏死因子α诱导的细胞凋亡的敏感性。
J Cell Sci. 2007 Jan 15;120(Pt 2):320-9. doi: 10.1242/jcs.03343. Epub 2007 Jan 2.
9
N-terminal residues in Cx43 and Cx40 determine physiological properties of gap junction channels, but do not influence heteromeric assembly with each other or with Cx26.Cx43和Cx40中的N端残基决定了间隙连接通道的生理特性，但不影响它们彼此之间或与Cx26的异聚体组装。
J Cell Sci. 2006 Jun 1;119(Pt 11):2258-68. doi: 10.1242/jcs.02953.

本文引用的文献

1
Loss of electrical communication, but not plaque formation, after mutations in the cytoplasmic loop of connexin43.连接蛋白43胞质环发生突变后，电信号通讯丧失，但斑块形成未受影响。
Heart Rhythm. 2004 Jul;1(2):227-33. doi: 10.1016/j.hrthm.2004.03.066.
2
Developmental regulation of the direct interaction between the intracellular loop of connexin 45.6 and the C terminus of major intrinsic protein (aquaporin-0).连接蛋白45.6细胞内环与主要内在蛋白（水通道蛋白-0）C端之间直接相互作用的发育调控
J Biol Chem. 2005 Jun 10;280(23):22081-90. doi: 10.1074/jbc.M414377200. Epub 2005 Mar 31.
3
Oculodentodigital dysplasia-causing connexin43 mutants are non-functional and exhibit dominant effects on wild-type connexin43.导致眼齿指发育异常的连接蛋白43突变体无功能，并对野生型连接蛋白43表现出显性效应。
J Biol Chem. 2005 Mar 25;280(12):11458-66. doi: 10.1074/jbc.M409564200. Epub 2005 Jan 10.
4
Dominant negative effect of connexin33 on gap junctional communication is mediated by connexin43 sequestration.连接蛋白33对缝隙连接通讯的显性负效应由连接蛋白43隔离介导。
J Cell Sci. 2004 Sep 15;117(Pt 20):4665-72. doi: 10.1242/jcs.01335. Epub 2004 Aug 25.
5
Adenoviral delivery of human connexin37 induces endothelial cell death through apoptosis.腺病毒介导的人连接蛋白37通过凋亡诱导内皮细胞死亡。
Biochem Biophys Res Commun. 2004 Jul 9;319(4):1144-51. doi: 10.1016/j.bbrc.2004.05.097.
6
Connexin43 and connexin26 form gap junctions, but not heteromeric channels in co-expressing cells.连接蛋白43和连接蛋白26形成间隙连接，但在共表达细胞中不形成异聚通道。
J Cell Sci. 2004 May 15;117(Pt 12):2469-80. doi: 10.1242/jcs.01084. Epub 2004 May 5.
7
Functional domain mapping and selective trans-dominant effects exhibited by Cx26 disease-causing mutations.Cx26致病突变所表现出的功能域映射和选择性反式显性效应。
J Biol Chem. 2004 Apr 30;279(18):19157-68. doi: 10.1074/jbc.M314117200. Epub 2004 Feb 19.
8
A carboxyl terminal domain of connexin43 is critical for gap junction plaque formation but not for homo- or hetero-oligomerization.连接蛋白43的羧基末端结构域对于间隙连接斑的形成至关重要，但对于同型或异型寡聚化并非如此。
Cell Commun Adhes. 2003 Jul-Dec;10(4-6):323-8. doi: 10.1080/15419060390263092.
9
Reduced gap junctional intercellular communication and altered biological effects in mouse osteoblast and rat liver oval cell lines transfected with dominant-negative connexin 43.用显性负性连接蛋白43转染的小鼠成骨细胞和大鼠肝卵圆细胞系中，间隙连接细胞间通讯减少及生物学效应改变。
Mol Carcinog. 2003 Aug;37(4):192-201. doi: 10.1002/mc.10137.
10
Specific amino-acid residues in the N-terminus and TM3 implicated in channel function and oligomerization compatibility of connexin43.连接蛋白43的N端和跨膜区3中与通道功能及寡聚化兼容性相关的特定氨基酸残基。
J Cell Sci. 2003 Aug 1;116(Pt 15):3189-201. doi: 10.1242/jcs.00604.

具有细胞质环缺失的连接蛋白43会抑制多种连接蛋白的功能。

Connexin43 with a cytoplasmic loop deletion inhibits the function of several connexins.

作者信息

Wang Min, Martínez Agustín D, Berthoud Viviana M, Seul Kyung H, Gemel Joanna, Valiunas Virginijus, Kumari Sindhu, Brink Peter R, Beyer Eric C

机构信息

Department of Pediatrics, Section of Hematology/Oncology and Stem Cell Transplantation, University of Chicago, Chicago, IL, USA.

出版信息

Biochem Biophys Res Commun. 2005 Aug 12;333(4):1185-93. doi: 10.1016/j.bbrc.2005.05.201.

DOI:10.1016/j.bbrc.2005.05.201
PMID:15979566
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2751629/
Abstract

Connexins (Cx) form gap junction channels mediating direct intercellular communication. To study the role of amino acids within the cytoplasmic loop, we produced a recombinant adenovirus containing Cx43 with a deletion of amino acids 130-136 (Cx43del(130-136)). Cx43del(130-136) expressed alone in HeLa cells localized within the cytoplasm and did not allow transfer of ions, neurobiotin or Lucifer yellow. When co-expressed with wild type Cx43, Cx43del(130-136) blocked electrical coupling and transfer of neurobiotin or Lucifer yellow. Cx43del(130-136) and Cx43 co-localized by immunofluorescence and were co-purified from Triton X-100-solubilized cell extracts. Intercellular transfer mediated by Cx37 and Cx45 (but not Cx26 or Cx40) was inhibited when co-expressed with Cx43del(130-136). Cx43del(130-136) co-localized with Cx37, Cx40, or Cx45, but not Cx26. These data suggest that Cx43del(130-136) produces connexin-specific inhibition of intercellular communication through formation of heteromeric connexons that are non-functional and/or retained in the cytoplasm.

摘要

连接蛋白（Cx）形成间隙连接通道，介导细胞间的直接通讯。为了研究细胞质环内氨基酸的作用，我们构建了一种重组腺病毒，其包含缺失氨基酸130 - 136的Cx43（Cx43del(130 - 136)）。单独在HeLa细胞中表达的Cx43del(130 - 136)定位于细胞质内，且不允许离子、神经生物素或荧光素黄的转运。当与野生型Cx43共表达时，Cx43del(130 - 136)阻断了电偶联以及神经生物素或荧光素黄的转运。Cx43del(130 - 136)和Cx43通过免疫荧光共定位，并从Triton X - 100溶解的细胞提取物中共同纯化出来。当与Cx43del(130 - 136)共表达时，由Cx37和Cx45介导的细胞间转运（但不是Cx26或Cx40介导的）受到抑制。Cx43del(130 - 136)与Cx37、Cx40或Cx45共定位，但不与Cx26共定位。这些数据表明，Cx43del(130 - 136)通过形成无功能和/或保留在细胞质中的异聚连接子，产生连接蛋白特异性的细胞间通讯抑制作用。