Rong Jun, Cheng Taipin, Liu Xiaona, Jiang Taozhen, Gu Hong, Zou Guolin
Department of Biochemistry, College of Life Science, Wuhan University, Wuhan, Hubei 430072, PR China.
Vaccine. 2005 Sep 23;23(40):4844-51. doi: 10.1016/j.vaccine.2005.05.015.
The objective of the present study was to investigate the feasibility of a subunit vaccine and a live bacteria vaccine to protect chickens against infectious bursal disease virus (IBDV) infection. The gene for VP2 of a new wild-type very virulent IBDV (vvIBDV) strain was cloned into an Escherichia coli expression system. Following expression, the recombinant VP2 and the induced expression bacteria were used to vaccinate chickens against virulent IBDV (vIBDV). Three weeks after the vaccination, chickens were inoculated with IBDV strain BC 6/85 by intranasal route or eyedrop route, prior to challenge anti-IBDV serum antibody was detected by AGP. All chickens vaccinated with recombinant VP2 could be detected anti-IBDV antibody. The subunit vaccine of recombinant VP2 conferred protection for 90--100% chickens, live bacteria vaccine of recombinant VP2 conferred protection for 85.7% chickens. The results indicate that E. coli BL 21/pET 28 a-VP2 could be used to develop recombinant VP2 vaccine against infectious bursal disease in chickens.
本研究的目的是探讨亚单位疫苗和活细菌疫苗保护鸡免受传染性法氏囊病病毒（IBDV）感染的可行性。将一株新的野生型超强毒IBDV（vvIBDV）毒株的VP2基因克隆到大肠杆菌表达系统中。表达后，用重组VP2和诱导表达的细菌对鸡进行接种，以抵抗强毒IBDV（vIBDV）。接种三周后，通过滴鼻或滴眼途径用IBDV毒株BC 6/85对鸡进行接种，在攻毒前通过琼脂扩散试验检测抗IBDV血清抗体。所有接种重组VP2的鸡均可检测到抗IBDV抗体。重组VP2亚单位疫苗对90% - 100%的鸡有保护作用，重组VP2活细菌疫苗对85.7%的鸡有保护作用。结果表明，大肠杆菌BL 21/pET 28 a-VP2可用于研制抗鸡传染性法氏囊病的重组VP2疫苗。
