Vanhoutte Tom, Huys Geert, De Brandt Evie, Fahey George C, Swings Jean
Laboratory of Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
FEMS Microbiol Lett. 2005 Aug 1;249(1):65-71. doi: 10.1016/j.femsle.2005.06.003.
The large intestine of dogs contains a complex microbial ecosystem with predominance of streptococci, bifidobacteria, lactobacilli, Bacteroides and Clostridium. Generally, this predominant microbiota in dogs is relatively stable in time but much less is known about its taxonomic composition. Moreover, almost no studies have been conducted to investigate this stability of the faecal microbial population in dogs upon prebiotic administration. The objective of the present study was to monitor possible changes in faecal microbiota of seven healthy adult dogs related to the administration of two fructans, oligofructose and inulin. For this purpose, population fingerprints generated by denaturing gradient gel electrophoresis (DGGE) analysis of universal V3 16 S rRNA gene PCR amplicons were compared between control (baseline) samples and samples collected after prebiotic feeding. From these DGGE gels, marked changes were observed in the faecal microbiota between subjects and before and after fructan administration. One DGGE band that appeared or intensified after fructan intake was further analyzed. Sequence analysis could attribute this band to a member of the Streptococcus bovis-equinus group. Following cultivation on MRS medium, a set of faecal isolates that most likely represent the stimulated streptococci were allocated to the species Streptococcus lutetiensis by (GTG)(5)-PCR fingerprinting and partial 16 S rRNA and sodA gene sequencing. The data provided in this study demonstrate the ability of fructans to influence the bacterial composition of the gut microbiota in healthy dogs. More work is needed to unravel the relevance of S. lutetiensis or other autochthonous organisms of the dog gut as target groups for prebiotic supplementation.
犬的大肠包含一个复杂的微生物生态系统，其中以链球菌、双歧杆菌、乳酸杆菌、拟杆菌和梭菌为主。一般来说，犬体内的这种优势微生物群在一段时间内相对稳定，但其分类组成却鲜为人知。此外，几乎没有研究探讨过益生元给药后犬粪便微生物群的这种稳定性。本研究的目的是监测7只健康成年犬粪便微生物群与两种果聚糖（低聚果糖和菊粉）给药相关的可能变化。为此，对通用V3 16 S rRNA基因PCR扩增子进行变性梯度凝胶电泳（DGGE）分析所产生的群体指纹图谱，在对照（基线）样本和益生元喂养后采集的样本之间进行了比较。从这些DGGE凝胶中观察到，不同个体之间以及果聚糖给药前后，粪便微生物群都有明显变化。对果聚糖摄入后出现或增强的一条DGGE条带进行了进一步分析。序列分析表明这条带属于牛-马链球菌群的一个成员。在MRS培养基上培养后，通过（GTG）（5）-PCR指纹图谱以及部分16 S rRNA和sodA基因测序，将一组最有可能代表受刺激链球菌的粪便分离株归为路氏链球菌。本研究提供的数据表明，果聚糖能够影响健康犬肠道微生物群的细菌组成。需要开展更多工作来阐明路氏链球菌或犬肠道其他本土微生物作为益生元补充目标群体的相关性。
