Jewell Dennis E, Jackson Matthew I, Cochrane Chun-Yen, Badri Dayakar V
Department of Grain Science and Industry, Kansas State University, Manhattan, KS 66506, USA.
Hill's Pet Nutrition, Inc., Topeka, KS 66617, USA.
Animals (Basel). 2022 Mar 2;12(5):627. doi: 10.3390/ani12050627.
This study assessed changes in canine fecal metabolites and microbiota with the consumption of foods with increasing concentrations of a fiber bundle including pecan shells, flax seed, and powders of cranberry, citrus, and beet that was previously shown (at 14% /) to improve stool quality, shift fecal bacterial metabolism from proteolysis to saccharolysis, increase abundance of saccharolytic bacteria, and decrease abundance of proteolytic bacteria. In this study, 48 healthy adult dogs were split evenly to consume different inclusion levels (0%, 1%, 2%, and 4%) of the fiber bundle for a 31-day period following a 28-day pre-feed period. Increases from baseline in the fecal short-chain fatty acids butyric acid, valeric acid, and hexanoic acid were observed only in the dogs that consumed the food with the 4% fiber bundle. With addition of any level of the fiber bundle, increases were seen in the polyphenols hesperidin, hesperetin, ponciretin, secoisolariciresinol diglucoside, secoisolariciresinol, and enterodiol. However, fecal microbiota and their metabolism, and stool scores were largely unaffected by the fiber bundle. Overall, addition of the fiber bundle appeared to increase bioactive metabolites of increased antioxidant and anti-inflammatory potency for beneficial to health and, at levels ≥4%, shifted gut bacterial metabolism toward saccharolysis.
本研究评估了犬类食用含有浓度递增的纤维束（包括山核桃壳、亚麻籽以及蔓越莓、柑橘和甜菜粉）的食物后粪便代谢物和微生物群的变化。先前的研究表明（含量为14%时），该纤维束可改善粪便质量，将粪便细菌代谢从蛋白水解转变为糖解，增加糖解细菌的丰度，并降低蛋白水解细菌的丰度。在本研究中，48只健康成年犬在28天的预饲喂期后，被平均分为四组，分别食用不同添加水平（0%、1%、2%和4%）的纤维束，为期31天。仅在食用含4%纤维束食物的犬只中观察到粪便短链脂肪酸丁酸、戊酸和己酸相对于基线水平有所增加。添加任何水平的纤维束后，多酚类物质橙皮苷、橙皮素、枳属苷、开环异落叶松脂素二葡萄糖苷、开环异落叶松脂素和肠二醇的含量均有所增加。然而，纤维束对粪便微生物群及其代谢以及粪便评分的影响不大。总体而言，添加纤维束似乎会增加具有更高抗氧化和抗炎效力的生物活性代谢物，对健康有益，且在纤维束含量≥4%时，会使肠道细菌代谢向糖解方向转变。
