Möbius C, Stein H J, Spiess C, Becker I, Feith M, Theisen J, Gais P, Jütting U, Siewert J R
Department of Surgery, Klinikum rechts der Isar der TU München, München, Germany.
Eur J Surg Oncol. 2005 Sep;31(7):755-9. doi: 10.1016/j.ejso.2005.01.006.
To examine COX2 expression and its relation to angiogenesis, Ki67 and Bcl2 expression in Barrett's cancer.
Specimens from 48 R0-resected Barrett's adenocarcinoma were immunostained for cyclooxygenase 2 (COX2), CD 31 and alpha-sm actin to discriminate between mature and immature vessels, Mib-1 and Bcl2. COX2 staining, angiogenesis, Ki67 expression and Bcl2 expression were also measured.
COX2 expression was increased in 25 of 48 cases. There was no significant correlation between COX2 expression and age, sex and tumor differentiation. A significant association was found between lymph node positive cases and elevated COX2 expression (p=0.008). The percentage of Ki67 positive cancer cells was 43.8% (range 15.4-67.5%) in the low COX2 group and 57.8% (range 12.0-84.6%) in the high COX2 group. The difference was statistically significant (p=0.046). The median neovascularisation coefficient in the low COX2 group was 11.68 (range 8.22-43.64) and 25.47 (range 8-38.3) in the high COX2 group. The difference was statistically significant (p=0.012). A significant difference in survival was observed between patients in the COX2 low category when compared with the COX2 high category (log-rank test p=0.013).
Elevated COX2 expression is associated with lymph-node metastases and reduced survival in Barrett's cancer. This appears to be related to the induction of angiogenesis and proliferation.
研究环氧化酶2（COX2）在巴雷特食管癌中的表达及其与血管生成、Ki67和Bcl2表达的关系。
对48例R0切除的巴雷特腺癌标本进行免疫染色，检测环氧化酶2（COX2）、CD 31和α平滑肌肌动蛋白，以区分成熟血管和未成熟血管，同时检测Mib-1和Bcl2。还测定了COX2染色、血管生成、Ki67表达和Bcl2表达。
48例中有25例COX2表达增加。COX2表达与年龄、性别和肿瘤分化之间无显著相关性。淋巴结阳性病例与COX2表达升高之间存在显著关联（p = 0.008）。低COX2组Ki67阳性癌细胞的百分比为43.8%（范围15.4 - 67.5%），高COX2组为57.8%（范围12.0 - 84.6%）。差异具有统计学意义（p = 0.046）。低COX2组的新生血管形成系数中位数为11.68（范围8.22 - 43.64），高COX2组为25.47（范围8 - 38.3）。差异具有统计学意义（p = 0.012）。与COX2高表达组相比，COX2低表达组患者的生存率存在显著差异（对数秩检验p = 0.013）。
COX2表达升高与巴雷特食管癌的淋巴结转移和生存率降低有关。这似乎与血管生成和增殖的诱导有关。
