Spadella C T, Breim L C, Mercadante M C, de Macedo C S, de Macedo A R
Department of Surgery, Universidade Estadual Paulista, School of Medicine, Botucatu, SP, Brasil.
Microsurgery. 1992;13(3):132-7. doi: 10.1002/micr.1920130307.
Outbred Wistar rats were randomly assigned to three experimental groups: GI, 10 nondiabetic control rats; GII, 10 alloxan-diabetic control rats; GIII, 25 alloxan-diabetic rats that received pancreaticoduodenal transplantation (PDT) from normal donor Wistar rats and were immunosuppressed with cyclosporin A. For 7 prior and 4, 7, 14, 21, and 30 days posttransplantation (during which the animals were housed in metabolic cages for periods of 24 hours) body weight, water and food intake, urine output, blood and urinary glucose, plasma insulin, and glucagon were recorded. These parameters were also concurrently recorded for diabetic and nondiabetic control rats. Animals were sacrificed after 30 days and histological and immunohistochemical studies of the pancreas were performed. Pancreatic transplants consistently and significantly improved the metabolic abnormalities of the diabetic rat (P less than 0.01) by restoring body weight gain, and by immediate relief of hyperglycemia, glucosuria, polyuria, polydipsia, and also the low levels of plasma insulin. The plasma glucagon, elevated in diabetic control rats, did not change after transplant.
远交系Wistar大鼠被随机分为三个实验组：GI组，10只非糖尿病对照大鼠；GII组，10只四氧嘧啶糖尿病对照大鼠；GIII组，25只接受来自正常供体Wistar大鼠的胰十二指肠移植（PDT）并用环孢素A免疫抑制的四氧嘧啶糖尿病大鼠。在移植前7天以及移植后4、7、14、21和30天（在此期间，动物被置于代谢笼中24小时），记录体重、水和食物摄入量、尿量、血糖和尿糖、血浆胰岛素和胰高血糖素。同时也对糖尿病和非糖尿病对照大鼠记录这些参数。30天后处死动物，并对胰腺进行组织学和免疫组织化学研究。胰腺移植通过恢复体重增加以及立即缓解高血糖、糖尿、多尿、烦渴以及血浆胰岛素水平低下，持续且显著地改善了糖尿病大鼠的代谢异常（P<0.01）。糖尿病对照大鼠中升高的血浆胰高血糖素在移植后未发生变化。
