Gordeev M I, Zvantsov A B, Goriacheva I I, Shaĭkevich E V, Ezhov M N
Med Parazitol (Mosk). 2005 Apr-Jun(2):4-5.
The paper describes the new species Anopheles artemievi belonging to a complex of the species maculipennis that may play an important role in the transmission of malaria in both the Fergana area and the Fergana valley. In terms of the morphological signs of imagoes, larvae, and the composition of the egg exochorion, the new species is the twin of An. sacharovi Favre and An. mihrtinius Shingarev. According to the pattern of polytene chromosomal disks, the described type is identical to An. maculipennis Meigen. The structure of the second internal transcribed spacer (ITS2) of a cluster of ribosomal genes in An. artemievi is species-specific and, in its nucleotide composition, differs from that in related species. The sequence of ITS2 in the new species has been registered at the GeneBank under No. AJ849886 and it is 419 p.n. The length of ITS2 in An. maculipennis and A. martinius is equal to 422 p.n. (AY238435) and 447 p.n. (AJ849885), respectively. The sequences of ITS in An. artemievi and An. maculipennis are 91% homologous; those in An. artemievi and An. martinius are 87% homologous. The nucleotide composition of ITS2 in An. artemievi differs from that in An. maculipennis in three deletions, 1 insertion, and 18 point substitutions and from that in An. martinius in 12 insertions, 2 deletions, and 40 point substitutions. The standard-type series is as follows: Kyrghyzstan, Batkensk Region, Leileksky district, Alga settlement, a cowshed, 1 male-holotype, l female-allotype. The paratypes are 2 males, 6 females.
该论文描述了新物种阿氏按蚊,它属于黄斑按蚊复合体,可能在费尔干纳地区和费尔干纳山谷的疟疾传播中发挥重要作用。就成虫、幼虫的形态特征以及卵外卵壳的组成而言,新物种是萨氏按蚊和米尔蒂尼按蚊的孪生种。根据多线染色体带型模式,所描述的类型与梅氏按蚊相同。阿氏按蚊核糖体基因簇的第二个内部转录间隔区(ITS2)的结构具有物种特异性,其核苷酸组成与相关物种不同。新物种的ITS2序列已在基因库中注册,登录号为AJ849886,长度为419个碱基对。梅氏按蚊和马丁按蚊的ITS2长度分别为422个碱基对(AY238435)和447个碱基对(AJ849885)。阿氏按蚊与梅氏按蚊的ITS序列同源性为91%;阿氏按蚊与马丁按蚊的ITS序列同源性为87%。阿氏按蚊的ITS2核苷酸组成与梅氏按蚊相比,有3处缺失、1处插入和18处点突变;与马丁按蚊相比,有12处插入、2处缺失和40处点突变。标准模式系列如下:吉尔吉斯斯坦,巴特肯斯克地区,莱列克区,阿尔加居民点,一个牛棚,1只雄性——正模,1只雌性——副模。副模为2只雄性,6只雌性。