Cardoso Paula de Carvalho, Lopes Guilherme Carpena, Vieira Luiz Clovis Cardoso, Baratieri Luiz Narciso
Department of Operative Dentistry, Federal University of Santa Catarina, Florianópolis, Brazil.
Oper Dent. 2005 May-Jun;30(3):376-81.
This study evaluated the effect of organic solvent (acetone or ethanol) on the microtensile bond strengths (MTBS) of an adhesive system applied to dry and moist dentin. Sixteen extracted human third molars were ground to expose a flat occlusal dentin surface and acid etched for 20 seconds (20% phosphoric acid gel, Gluma Etch 20 Gel, Heraeus/Kulzer). After rinsing the acid etchant, an ethanol-based one-bottle adhesive system was applied to the mesial half of the occlusal dentin surface. An acetone-based, one-bottle adhesive system was applied to the distal half of the ground dentin surface. The teeth were randomly assigned to groups. In Group 1, the etched dentin was thoroughly air dried and an ethanol-based one-bottle adhesive system was applied (Gluma Comfort Bond, Heraeus/Kulzer) (GCB). In Group 2, the etched dentin was thoroughly air dried and an acetone-based one-bottle adhesive system was applied (Gluma One Bond, Heraeus/Kulzer)(GOB). In Group 3, excess moisture was removed after acid etching, leaving a moist dentin surface and a one-bottle ethanol-based adhesive was applied (Gluma Comfort Bond). In Group 4, excess moisture was removed after acid etching, leaving a moist dentin surface and an acetone-based adhesive was applied (Gluma One Bond). A hybrid resin composite (Venus, Heraeus/Kulzer) was applied to the bonded surface in four 1-mm increments and light cured according to manufacturer's directions. The specimens were then sectioned with a slow-speed diamond saw in two perpendicular directions to obtain sticks with a cross-section of 0.5 +/- 0.05 mm2. The microtensile bond strength (MTBS) test was performed with a Bencor device in an Instron machine at a crosshead speed of 0.5 mm/minute. The data were subjected to a two-way ANOVA and Scheffé Post hoc test (p < 0.05). The experimental MTBS measured for dry dentin were Group 1 = 37.0 +/- 10.6 and Group 2 = 34.7 +/- 9.0 in MPa (mean +/- SD); and on moist dentin, Group 3 = 50.7 +/- 11.0 and Group 4 = 38.5 +/- 10.5 in MPa (mean +/- SD). The ethanol based adhesives resulted in higher MTBS than acetone-based adhesive (p < 0.008) and bonding to moist dentin resulted in higher MTBS (p < 0.001). GCB applied on moist dentin resulted in statistically higher bond strengths than the other groups. The highest MTBS were achieved with the use of an ethanol-based adhesive to moist dentin.
本研究评估了有机溶剂（丙酮或乙醇）对应用于干燥和潮湿牙本质的粘结系统的微拉伸粘结强度（MTBS）的影响。选取16颗拔除的人类第三磨牙，磨平使其咬合面牙本质暴露，用20%磷酸凝胶（Gluma Etch 20 Gel，贺利氏/库尔泽公司）酸蚀20秒。冲洗酸蚀剂后，将乙醇基单瓶粘结系统应用于咬合面牙本质表面的近中半侧。将丙酮基单瓶粘结系统应用于磨除牙本质表面的远中半侧。牙齿随机分组。第1组，酸蚀后的牙本质彻底吹干，应用乙醇基单瓶粘结系统（Gluma Comfort Bond，贺利氏/库尔泽公司）（GCB）。第2组，酸蚀后的牙本质彻底吹干，应用丙酮基单瓶粘结系统（Gluma One Bond，贺利氏/库尔泽公司）（GOB）。第3组，酸蚀后去除多余水分，保留潮湿牙本质表面，应用乙醇基单瓶粘结剂（Gluma Comfort Bond）。第4组，酸蚀后去除多余水分，保留潮湿牙本质表面，应用丙酮基粘结剂（Gluma One Bond）。将一种混合树脂复合材料（Venus，贺利氏/库尔泽公司）以四个1毫米的增量应用于粘结表面，并按照制造商的说明进行光固化。然后用低速金刚石锯在两个垂直方向对标本进行切片，以获得横截面为0.5±0.05平方毫米的小棒。使用Bencor装置在Instron机器上以0.5毫米/分钟的十字头速度进行微拉伸粘结强度（MTBS）测试。数据进行双向方差分析和谢费尔事后检验（p<0.05）。干燥牙本质的实验MTBS测量值为：第1组=37.0±10.6，第2组=34.7±9.0，单位为兆帕（平均值±标准差）；在潮湿牙本质上，第3组=50.7±11.0，第4组=38.5±10.5，单位为兆帕（平均值±标准差）。乙醇基粘结剂产生的MTBS高于丙酮基粘结剂（p<0.008），粘结到潮湿牙本质上产生的MTBS更高（p<0.001）。应用于潮湿牙本质的GCB产生的粘结强度在统计学上高于其他组。使用乙醇基粘结剂粘结潮湿牙本质时获得了最高的MTBS。
