使用固定化多胺氧化酶（土曲霉）的柱后反应器，随后进行电化学检测，对亚精胺和精胺进行高效液相色谱分析。
High performance liquid chromatography of spermidine and spermine using a postcolumn reactor of immobilized polyamine oxidase (Aspergillus terreus) followed by electrochemical detection.
作者信息
Watanabe N
机构信息
Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan.
出版信息
Biomed Chromatogr. 1992 Jan-Feb;6(1):1-3. doi: 10.1002/bmc.1130060102.
PMID:1600367
Abstract
A highly sensitive and selective method for the determination of spermidine and spermine has been developed. A polyamine oxidase (Aspergillus terreus) immobilized column was used as a postcolumn reactor. The detection limit was 0.2 pmol/injection for both spermidine and spermine with a linear range of three orders of magnitude.
摘要
已开发出一种用于测定亚精胺和精胺的高灵敏度和高选择性方法。使用固定化多胺氧化酶（土曲霉）柱作为柱后反应器。亚精胺和精胺的检测限均为0.2 pmol/进样，线性范围为三个数量级。
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