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[蛋白质作为具有不同形态发生潜力的苦荞麦(鞑靼荞麦)愈伤组织培养中的形态发生标记]

[Proteins as morphogenetic markers in callus cultures of buckwheat Fagopyrum tataricum (L.) Gaertn with different morphogenetic potential].

作者信息

Maksiutova N N, Galeeva E I, Rumiantseva N I, Viktorova L V

出版信息

Izv Akad Nauk Ser Biol. 2005 May-Jun(3):306-10.

Abstract

The content of soluble proteins and individual polypeptides was studied in calluses of buckwheat Fagopyrum tataricum (L.) Gaertn with different morphogenic potential. The morphogenic callus had a higher content of soluble proteins and cyclic pattern of changes in this index during passaging, which seems to be due to formation of proembryogenic cell complexes. Comparison of the protein patterns of the calluses demonstrated differences in composition and content of individual components. Morphogenic (35 and 73 kDa) and nonmorphogenic callus-specific proteins (16 and 62 kDa) have been revealed.

摘要

对具有不同形态发生潜力的苦荞麦(鞑靼荞麦)愈伤组织中可溶性蛋白质和单个多肽的含量进行了研究。形态发生愈伤组织中可溶性蛋白质含量较高,且在继代培养过程中该指标呈周期性变化,这似乎是由于原胚性细胞复合体的形成。愈伤组织蛋白质图谱的比较表明,各个成分的组成和含量存在差异。已发现形态发生愈伤组织特异性蛋白质(35 kDa和73 kDa)和非形态发生愈伤组织特异性蛋白质(16 kDa和62 kDa)。

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